1996

Pella, Iowa

Variety:  Prairie Rose (Wilson Seeds)
Seeding date:  spring, 1994 (1996 was the second year of harvest)

Vitazyme application:  At spring greenup (Apr 19), Vitazyme was sprayed on the leaves and soil at 13 oz/acre to the treated half of the field.  About 7 to 10 days after the first cutting, a second application of 13 oz/acre was made.

Alfalfa yield (first cutting):
Control: 2.01 tons/acre
Vitazyme: 2.56 tons/acre
(+27%)

Alfalfa yield (second cutting):
Control: 1.46 tons/acre
Vitazyme: 1.73 tons/acre
(+19%)

Fertilization:  unknown

1997

Prineville, OR (Oregon State University)

Variety:  WL-252HQ
Crop stage:  established
Harvest date:  Oct 15, 1997

Vitazyme application:  Vitazyme at 13 oz/acre sprayed over the plants and soil at spring greenup (May 6), and after each cutting

Forage Protein (first cutting):
Control: 19.2%
Vitazyme: 20.3*%
(+1.1 percentage points)

Fertilization:  unknown

1999

Tracy, CA

Variety:  Yolo
Cutting:  fourth

Vitazyme application:  Vitazyme at 13 oz/acre by air to the new growth, shortly after the third cutting had been harvested

Acid detergent fiber:
Control: 30.6%
Vitazyme: 30.5

Crude Protein (first cutting):
Control: 22.1%
Vitazyme: 23.2%
(+1.1 percentage points)

Total digestible nutrients:
Control: 59.4%
Vitazyme: 59.5%

Net energy of lactation, Mcal/lb:
Control: 0.605 Mcal/lb
Vitazyme: 0.606 Mcal/lb

Fertilization:  none

2000

Cedar Falls, IA

Variety:  Viking
Planting date:  Apr 28, 1999
Row spacing:  7 inches
Seeding rate:  16 lb/acre
Soil type:  Aredale loam
Harvest dates: 
Jun 8, Jul 13, Oct 12, 2000

Vitazyme application:  In 1999: Vitazyme at 13 oz/acre on Treatments 1 and 3 after planting

2000: Vitazyme at spring greenup (April 26) on treatments 2 and 3.

Vitazyme after cut 1 (Jun 6) on treatment 3.

Vitazyme after cut 2 (Jul 18) on treatment 3.

Forage yield (first cutting):
Control: 0.805 tons/acre
Vitazyme, one time: 0.805 tons/acre
Vitazyme, three times: 0.767 tons/acre

Forage yield (second cutting):
Control: 1.830 tons/acre
Vitazyme, one time: 1.955 tons/acre*
Vitazyme, three times: 2.030 tons/acre*

*Significantly greater than the control at P=0.10 according to Duncan's New Multiple Range Test.  LSD_0.10=0.168.

Forage yield (third cutting):
Control: 0.982 tons/acre
Vitazyme, one time: 1.035 tons/acre*
Vitazyme, three times: 1.128 tons/acre*

*Significantly greater than the control at P=0.10 according to Duncan's New Multiple Range Test.  LSD_0.10=0.33.

Forage quality effects:
Vitazyme in all cases improved protein content, relative feeding value, net energy, and phosphorus levels in the forage.

Fertilization:  none

2000

Cedar Falls, IA

Variety:  unknown
Soil type:  Maxfield silt loam, surface soil
Tray size and type:  10 x 21 inches, slotted tray inside a  solid tray, and a paper liner below

Vitazyme application:  The equivalent of 13 oz/acre Vitazyme was applied to the soil surface at planting.

Emerged plants on day 7:
Control: 27.5
Vitazyme: 29.5

Plant vigor:
Vitazyme significantly improved plant vigor over the control by 11 days after planting.

Soil and plant water retention:
Vitazyme caused more soil and plant water retention than the control at all times up to 13 days after planting.

Fertilization:  none

2002

Arkport, NY (Stutzman's Research Farm)

Variety:  Doebler's EX 468
Planting date:  Jul 1, 2002
Soil type:  silt loam
Harvest date:  Oct 1, 2002

Vitazyme application:  13 oz/acre to the seeds and soil before the seeds were rolled into the soil

Dry yield:
Control: 0.878 tons/acre
Vitazyme: 0.970 tons/acre*
(+10%)

*Significantly greater than the control at P=0.04 according to the Tukey-Kramer Test

Milk production (University of Wisconsin Milk 2000 calculations):
Control: 2,290 lb/acre
Vitazyme: 2,519 lb/acre
(+10%)

*Significantly greater than the control at P=0.10 according to the Tukey-Kramer Test

Increase in milk income:
+$34.35/acre

Fertilization:  none

2002

Arkport, NY (Stutzman's Research Farm)

Variety:  Doebler's Phirst
Planting date:  Jun 10, 2002
Soil type:  silt loam
Harvest date:  Oct 1, 2002

Vitazyme application:  13 oz/acre on the leaves and soil at 3 to 4-inch height of regrowth, on August 15

Fresh yield:
Control: 2.95 tons/acre
Vitazyme: 3.23 tons/acre
(+9%)

Dry yield:
Control: 0.732 tons/acre
Vitazyme: 0.834 tons/acre
(+14%)

Increase in milk income:
+$40.20/acre

Fertilization:  none

2004

Gladewater, TX (Vital Earth Resources Research Greenhouse)

Variety:  common
Planting date:  Dec 30, 2002
Soil type:  fine sandy clay loam
Planting rate:  thinned to 12 plants/pot
Pot size:  1 gallon

Vitazyme and Rhizobium application:  Treatment 2, a slurry was made of the rhizobium and alfalfa seeds, and soaked for 1 hour before planting; Treatment 3, alfalfa seeds were soaked for 1 hour in undiluted Vitazyme before planting; Treatment 4, alfalfa seeds were soaked with undiluted Vitazyme and rhizobium for 1 hour before planting.

Plant height:
Control: 15.7 cm
Rhizobium: 20.6 cm*
Vitazyme: 17.4 cm
Vitazyme + Rhizobium: 21.6 cm*
(+38%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=3.1 cm.

Leaf chlorophyll:
Control: 23.6 SPAD units
Rhizobium: 34.9 a SPAD units*
Vitazyme: 21.4 SPAD units
Vitazyme + Rhizobium: 34.2 SPAD units*
(+45%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=3.9 SPAD units.

Nodule weight:
Control: 0.20 g/pot
Rhizobium: 0.26 g/pot
Vitazyme: 0.12 g/pot
Vitazyme + Rhizobium: 0.48 g/pt*
(+140%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=0.19 g.

Dry weight:
Control: 4.22 g/pot
Rhizobium: 5.47 g/pot*
Vitazyme: 4.79 g/pot
Vitazyme + Rhizobium: 7.08 g/pt*
(+68%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=1.21 g.

Fertilization:  none