Date

Location

Cultural System

Vitazyme/Fertilization

Results

1996

Albion, New York

Variety:  Histyle for processing

Seeding date:  June 1, 1996

Seeding rate:  120,000 seeds/acre

Row spacing:  30 inches

Previous crops:  field corn

Harvest date:  July 30, 1996

Vitazyme application:  Vitazyme at 12 oz/acre, sprayed on the soil at planting

Snap Bean Yield:

Control: 6,220 bu/acre
Vitazyme: 7,130 bu/acre (+15%)

Income Increase:

+$59.15/acre

Fertilization:  The control area received 1 ton/acre of lime, before planting, and 350 lb/acre of 10-26-10 (%N-P₂O₅-K₂O) at planting.  The Vitazyme area received a preplant application of 400 lb/acre high calcium lime, 200 lb/acre of ammnium sulfate, and 1 gal/acre of Ca(NO₃)₂ + sugar.  The Vitazyme area also received 20 lb/acre of 10-26-10 at planting.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Gladewater, Texas

(Vital Earth Resources Research Greenhouse)

Variety:  bush type of snap beans

Planting date:  February 18, 1999

Pot size:  one gallon

Soil type:  sandy loam

Harvest date:  March 25, 1999

Vitazyme application:  13 oz/acre

Snap bean yield:

Vitazyme + AB-1: 6.50 g/pot (a) (+16%)

BG-1: 5.93 g/pot (ab) (+6%)

Vitazyme: 5.74 g/pot (b) (+3%)

BP-1: 5.73 g/pot (b) (+3%)

AB-1: 5.72 g/pot (b) (+2%)

Control: 5.59 g/pot (bc)

Vitazyme + DLM4: 5.04 g/pot (c) (-10%)

Means followed by the same letter are not significantly different according to the Student-Newman-Keuls Test (P=0.10).

Fertilization:  none

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Fayetteville, Arkansas

(University of Arkansas)

Tifton, Georgia

(University of Georgia)

West Lafayette, Indiana

(Purdue University)

Mississippi State, Mississippi

(Mississippi State University)

Baton Rouge, Louisiana

(Louisiana State University)

Raleigh, North Carolina

(North Carolina State University)

Plano, Texas

(Gustafson)

Knoxville, Tennessee

(University of Tennessee)

Charleston, South Carolina

(Clemson University)

Variety:  unknown

Replications:  four

Vitazyme application:  (1) Lot 1.  Regular Vitazyme (10%), with seeds soaked for five minutes and then air dried.

(2) Lot 2.  T-22 (1 oz/gallon of soluble powder), with seds soaked for five minutes and then dried.

(3) Lot 3.  Vitazyme (10%) + T-22 (1 oz/gallon), with seds soaked for five minutes and tehn dried.

(4) Lot 4.  Autoclaved Vitazyme (10%), with seeds soaked for five minutes and then air dried.  Autoclaving was done at 15 lb/in² pressure for 15 min at 121 C, and reautoclaved one day later.

Arkansas (University of Arkansas)

Number of plants:

Control: 71.6

Vitazyme, regular: 75.0 (+5%)

Vitazyme, autoclaved: 74.8 (+4%)

Georgia (University of Georgia)

Number of plants:

Control: 62.2

Vitazyme, regular: 70.0 (+13%)

Vitazyme, autoclaved: 75.8* (+22%)

*Significantly greater than the control at P=0.06.

Indiana (Purdue University)

Number of plants:

Control: 63.8

Vitazyme, regular: 63.8 (0%)

Vitazyme, autoclaved: 62.2 (-3%)

Mississippi (Mississippi State University)

Number of plants:

Control: 85.6

Vitazyme, regular: 88.4 (+3%)

Vitazyme, autoclaved: 88.4 (+3%)

Dry matter yield, grams per 10 plants:

Control: 221.71

Vitazyme, regular: 259.32 (+17%)

Vitazyme, autoclaved: 248.26 (+12%)

Louisiana (Louisiana State University)

Number of plants:

Control: 26.0

Vitazyme, regular: 42.8 (+65%)

Vitazyme, autoclaved: 31.4 (+21%)

*Significantly greater than the control at P=0.05.

North Carolina (North Carolina State University)

Number of plants:

Control: 81.6

Vitazyme, regular: 82.4 (+1%)

Vitazyme, autoclaved: 83.0 (+2%)

Texas (Gustafson)

Number of plants:

Control: 70.6

Vitazyme, regular: 72.9 (+3%)

Vitazyme, autoclaved: 75.2* (+7%)

*Significantly greater than the control at P=0.01.

Tennessee (University of Tennessee)

Number of plants:

Control: 30.2

Vitazyme, regular: 38.4 (+27%)

Vitazyme, autoclaved: 44.0* (+46%)

*Significantly greater than the control at P=0.07.

South Carolina (Clemson University)

Number of plants:

Control: 88.6

Vitazyme, regular: 89.0 (+0.5%)

Vitazyme, autoclaved: 90.4 (+2%)

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Gladewater, Texas

(Vital Earth Resources Research Greenhouse)

Variety:  Kentucky Wonder

Soil type:  Bowie very fine sandy loam

Planting rate:  7 seeds/pot, thinned to 3 plants/pot

Pot size:  one gallon

Vitazyme application:  100 ml of a 0.002% solution applied over the soil surface after planting;  control plots received 100 ml of water only

Plant dry weight:

Control: 3.46 grams
Vitazyme: 5.28 grams* (+53%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=0.42 g.

Plant height:

Control: 33.8 cm
Vitazyme: 39.5 cm* (+17%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=0.2.2 cm.

Number of beans and blossoms:

Control: 7.3
Vitazyme: 15.8* (+116%)

*Significantly greater than the control at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.10=2.8.

Fertilization:  none

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Mississippi State, Mississippi

(Mississippi State University)

 Knoxville, Tennessee

(University of Tennessee)

Variety:  unknown

Row length:  30 to 40 feet

Growth period:  28 days

Vitazyme application:

(1) Lot 1.  Regular Vitazyme (10%), with seeds soaked for five minutes and then dried.

(2) Lot 2.  T-22 (1 oz/gallon of soluble powder), with seeds soaked for five minutes and then dried.

(2) Lot 3.  Vitazyme (10%) + T-22 (1 oz/gallon), with seeds soaked for five minutes and then dried.

(2) Lot 4.  Vitazyme autoclaved (10%), with seeds soaked for five minutes and then dried.

T-22 (Trichoderma harzianum, strain T-22) application:  see above

Mississippi (Mississippi State University)

Number of plants:

Control: 88.2
Vitazyme: 85.8 (-3%)

T-22: 89.0 (+1%)

Vitazyme + T-22: 91.8 (+4%)

Vitazyme: 88.0 (0%)

Tennessee (University of Tennessee)

Number of plants:

Control: 66.6
Vitazyme: 71.0 (+7%)

T-22: 83.4 (+25%)

Vitazyme + T-22: 68.4 (+3%)

Vitazyme: 68.2 (+2%)

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Gladewater, Texas (Vital Earth Resources Research Center)

Variety:  Kentucky Wonder

Planting date:  November 21, 2001

Soil type:  Bowie very fine sandy loam

Population:  7 seeds/pot, thinned to 3/pot

Pot type:  1 gallon

Harvest date:  January 8, 2002

Vitazyme application:  100 ml of a 0.01% Vitazyme solution applied to the soil surface of each pot

Plant height:

Control: 31.0 cm
Vitazyme*: 33.5 cm (+8%)
*Significantly different at P=0.10, according to the Tukey Kramer Test.  LSD0.10=1.5 cm
 

Dry weight:

Control: 3.64 grams
Vitazyme: 4.01 grams (+10%)

*Significantly different at P=0.10, according to the Tukey Kramer Test.  LSD0.10=0.38 grams

Fertilization:  none

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Byron, New York

(New York Crop Research Facility - A.C.D.S. Research)

Variety:  Histyle

Planting date:  June 28, 2003

Seeding rate:  unknown (drilled)

Soil type:  Galen very fine sandy loam

Tillage:  conventional

Row spacing:  30 inches

Vitazyme application:  (1) 13 oz/acre on the seeds at planting (2) 13 oz/acre on the leaves and soil shortly before bloom

Snap bean yield:

Control: 5.63 tons/acre
Vitazyme: 5.80 tons/acre (+3%)

Plant height at 28 days:

Control: 7.53 inches
Vitazyme: 7.60 inches (+0.07 inches)

Bean size, sieve size 5:

Control: 31%
Vitazyme: 33% (+2 percentage points)

Fertilization:  Both the Vitazyme and control treatments received 300 lb/acre of a 15-15-15 N-P₂O₅-K₂O fertilizer sidedressed.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Batavia,  New York

(New York Crop Research Facility - A.C.D.S. Research)

Variety:  Histyle

Planting date:  June 20, 2003

Row spacing:  30 inches

Soil type:  Cazenovia silt loam

Planting depth:  1.5 inches

Row spacing:  30 inches

Previous crop:  sweet corn

Replications:  four

Harvest date:  August 12, 2003

Vitazyme application:  A starter treatment of 13 oz/acre was applied on the bean seeds at planting for Treatment 4, and with Na-churs Alpine 6-24-6 starter fertilizer for Treatment 5.

Bean yield increase with Vitazyme:

Control: 2.03 tons/acre (b)

Vitazyme: 2.47 tons/acre (a) (+22%)

Means followed by the same letter are not significantly different according to Bartlett's Test (P=0.05).  LSD (0.05)=0.23 ton/acre.

Income increase with Vitazyme:

+$55.68/acre

Fertilization:  All starter fertilizers and biostimulant products were applied at the rates prescribed by the manufacturer, and either on the seeds or in a 2 x 2 placement (inches beside and below the seeds).

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Batavia,  New York

(New York Crop Research Facility - A.C.D.S. Research)

Variety:  Histyle

Planting date:  June 28, 2003

Row spacing:  30 inches

Soil type:  Cazenovia silt loam

Planting depth:  1.5 inches

Row spacing:  30 inches

Previous crop:  sweet corn

Replications:  four

Harvest date:  August 27, 2003

Vitazyme application:  A starter treatment of 13 oz/acre was applied on the bean seeds at planting for Treatment 4, and with Na-churs Alpine 6-24-6 starter fertilizer for Treatment 5.

Bean yield increase with Vitazyme:

Control: 2.61 tons/acre (b)

Vitazyme: 3.30 tons/acre (a) (+23%)

Vitazyme + 6-24-6: 3.34 tons/acre (a) (+24%)
6-24-6: 3.26 tons/acre (a) (+21%)

Means followed by the same letter are not significantly different according to Bartlett's Test (P=0.05).  LSD (0.05)=0.19 ton/acre.

Income increase with Vitazyme alone:

+$81.70/acre

Fertilization:  All starter fertilizers and biostimulant products were applied at the rates prescribed by the manufacturer, and either on the seeds or in a 2 x 2 placement (inches beside and below the seeds).