Date

Location

Cultural System

Vitazyme/Fertilization

Results

1996

Middle Point, Ohio

Variety:  Pioneer 3394

Planting date:  May 20, 1996

Soil type:  silty clay

Population:  27,000 seeds/acre

Harvest date:  November 13, 1996

Vitazyme application: Vitazyme at 13 oz/acre injected on the seeds at planting, mixed with starter fertilizer

Moisture, at harvest:

Control: 14.9%
Vitazyme: 12.3% (-2.6 percentage points)
 

Dry Matter, at harvest:

Control: 85.1%
Vitazyme: 87.7% (+2.6 percentage points)

Crude Protein, (dry):

Control: 9.2%
Vitazyme: 9.3% (+0.1 percentage point)

Acid Detergent Fiber:

Control: 3.5%
Vitazyme: 5.4% (+54%)

Total Digestible Nutrients:

Control: 88.0%
Vitazyme: 87.0% (-1%)

Ash:

Control: 1.46%
Vitazyme: 1.70% (+16%)

Plant observations on August 1, 1996: On August 1, the root mass of the plants was much greater for the Vitazyme treatment, and the leaf size, plant height, and ear size were greater versus the control.  Leaf chlorophyll was 3.8 SPAD units higher for the Vitazyme treated plants than for the control.

Fertilization:  At planting, 5 gal/acre of 2-20-18 (%N-P₂O₅-K₂O) + sugar + 1.7 pt/acre Zn + 10 pt/acre Mn were applied directly on the seeds.  A 10 gal/acre of a 28% N + 5%S + sugar solution were applied to the side and below the seed row.  At 12 to 18 inches height, 32 gal/acre of 28% N was knifed in between the rows.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Arkport, New York

(Stutzman Research Farm)

Variety:  unknown

Planting date:  unknown

Soil type:  silt loam

Seeding rate:  32,000 seeds/acre

Row spacing:  30 inches

Harvest date:  unknown

Replicates:  four

Vitazyme application:  13 oz/acre on the leaves and soil at 15 inches plant height

Leaf chlorophyll:

Control: 56.3 SPAD units
Vitazyme: 59.0 SPAD units (+2.7 SPAD units)

Dry matter %:

Control: 24.3%
Vitazyme: 29.2% (+20%)
 

Silage yield:

Control: 25.90 tons/acre
Vitazyme: 29.45 tons/acre (+14%)

Dry matter digestibility*:

Control: 48.23%
Vitazyme: 69.52% (+44%)

*The % of silage digested by the cow.

Neutral detergent fiber*:

Control: 57.0%
Vitazyme: 38.5% (-32%)

*Based on an as-fed moist basis..

DNDF,DM*:

Control: 39.58 %
Vitazyme: 40.86% (+3%)

*The digestible portion of the plant less the grain (vegetative portion only), expressed in the terms of dry matter.

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Arkport, New York

(Stutzman Research Farm)

Variety:  Golden Harvest 7651 Roundup Ready

Planting date:  May 25, 2000

Soil type:  silt loam

Seeding rate:  32,000 seeds/acre

Row spacing:  30 inches

Replicates:  four

Harvest date:  September 27, 2000

Vitazyme application:  Treatment 2: 13 oz/acre in the seed row at planting; Treatment 3: 13 oz/acre sprayed on the leaves and soil at 15 inches corn height

Silage yield:

Control: 27.38 tons/acre
Vitazyme in furrow: 29.88 tons/acre (+9%)

Vitazyme at 15 in: 27.58 tons/acre

*Means followed by the same letter are not significantly different at P=0.06, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 1.77 cm.

Dry matter moisture:

Control: 28.13% H₂O (a)
Vitazyme in furrow: 29.52 H₂O (b) (+5%)

Vitazyme at 15 in: 29.22 H₂O (ab) (+4%)

*Means followed by the same letter are not significantly different at P=0.06, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 0.90.
 

NDF, DM*:

Control: 44.15% (b)
Vitazyme in furrow: 44.45% (a) (+1%)

Vitazyme at 15 in: 44.43% (a) (+1%)

*NDF, DM = neutral detergent fiber, expressed in terms of dry matter

Means followed by the same letter are not significantly different at P=0.10, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 0.32.

NDF, as fed*:

Control: 12.45% (b)
Vitazyme in furrow: 13.10% (a) (+5%)

Vitazyme at 15 in: 12.93% (ab) (+4%)

*NDF, as fed = neutral detergent fiber, on an as-fed moist basis

Means followed by the same letter are not significantly different at P=0.12, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 0.85.

IVTD, DM*:

Control: 81.10% (b)
Vitazyme in furrow: 83.15% (a) (+3%)

Vitazyme at 15 in: 82.90% (a) (+2%)

*IVTD, DM = in vitro true digestibility, expressed in terms of dry matter.  It is an anaerobic fermentation performed in the laboratory using rumen fluid from cows consuming a typical ration.

Means followed by the same letter are not significantly different at P=0.04, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 0.1.28.

DNDF, DM*:

Control: 57.18% (b)
Vitazyme in furrow: 61.98% (ab) (+8%)

Vitazyme at 15 in: 66.60% (a) (+16%)

*DNDF, DM = the digestible portion of the plant les the grain (vegetation portion portion only); expressed in terms of dry matter

Means followed by the same letter are not significantly different at P=0.10, according to Tukey's Honestly Significant Difference Test.  LSD_0.10 = 9.43..

Income return per acre:

Control: $7,062/acre
Vitazyme in furrow: $7,970/acre (+$908/acre)

Vitazyme at 15 in: $7,302/acre (+$240/acre)

Fertilization:  175 lb/acre N and 120 lb/acre K₂O preplant incorporated and sidedressed, plus 100 lb/acre 5-24-25-micronutrients starter at planting

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Blue Grass, Iowa

Variety:  Baldridge 705 silage corn

Planting date:  May 1, 2001

Soil type:  unknown

Seeding rate:  28,000 seeds/acre

Row spacing:  30 inches

Previous:  corn

Harvest date:  unknown

Vitazyme and Grozyme applications:  12 oz/acre of Vitazyme with the herbicide for Treatment 3; 12 oz/acre of Grozyme with the herbicide for Treatment 2

Only quality parameters were determined in this study.

Crude protein, %:

Control: 7.8%
Ag Spectrum Program: 8.4%

Vitazyme: 8.3%

Neutral detergent fiber, %:

Control: 40.1%
Ag Spectrum Program: 42.3%

Vitazyme: 47.0%

Acid detergent fiber, %:

Control: 21.8%
Ag Spectrum Program: 23.2%

Vitazyme: 26.8%

Phosphorus, %:

Control: 0.22%
Ag Spectrum Program: 0.22%

Vitazyme: 0.23%

Calcium, %:

Control: 0.31%
Ag Spectrum Program: 0.38%

Vitazyme: 0.45%

Potassium, %:

Control: 0.74%
Ag Spectrum Program: 0.99%

Vitazyme: 1.08%

Magnesium, %:

Control: 0.20%
Ag Spectrum Program: 0.23%

Vitazyme: 0.26%

Fertilization:  All areas received 100 lb/acre N plus 5 lb/acre sulfur at planting.  All areas also received 50 lb/acre of additional N sidedressed, with 5 lb/acre of sulfur.  Treatments 1 and 3 received 5 gal/acre of Liquid Grow 8-19-3 starter in the furrow at planting, while Treatment 2 received 5 gal/acre of Ag Spectrum Clean Start + Kickoff in the furrow at planting.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Arkport, New York

(Stutzman Research Farm)

Variety:  Mycogen 108

Planting date:  May 22, 2000

Soil type:  silt loam

Seeding rate:  30,000 seeds/acre

Row spacing:  30 inches

Replicates:  three

Harvest date:  September 11, 2001

Vitazyme application:  Treatments 2, 4, and 5 received 13 oz/acre of Vitazyme directly on the seed at planting, while Treatments 4 and 5 were given an additional 13 oz/acre sprayed over the plants and soil at 20 inches in plant height.  Treatment 3 received 13 oz/acre of Vitazyme in the dry fertilizer, placed 2 inches below and 2 inches beside the seed row.

Silage yield*:

1. Control: 17.07 tons/acre

2. Vitazyme on seed + 100% N: 18.90 tons/acre (+11%)

3. Vitazyme in fertilizer: 19.40 tons/acre (+14%)

4. Vitazyme on seed + leaves + 100% N: 18.13 tons/acre (+6%)

5. Vitazyme on seed + leaves + 70% N: 18.03 tons/acre (+6%)
*Adjusted yield to 32% dry matter.  Yields are not significantly different at P=0.10, but Treatment 3 is greater than the control at P=0.12 according to the Tukey-Kramer Test.  LSD_0.1=2.63 tons/acre

Crude protein (% of dry matter):

1. Control: 7.77%

2. Vitazyme on seed + 100% N: 8.17%

3. Vitazyme in fertilizer: 7.77%

4. Vitazyme on seed + leaves + 100% N: 6.97%

5. Vitazyme on seed + leaves + 70% N: 8.37%
LSD_0.1=0.93%

Trt. 5>Trt. 4 at P=0.10 (Tukey-Kramer)

Acid detergent insoluble crude protein* (% of dry matter):

1. Control: 0.67%

2. Vitazyme on seed + 100% N: 0.57%

3. Vitazyme in fertilizer: 0.40%

4. Vitazyme on seed + leaves + 100% N: 0.73%

5. Vitazyme on seed + leaves + 70% N: 0.63%
*Bound or unavailable protein

LSD_0.1=0.29%

Trt. 4>Trt. 3 at P=0.10 (Tukey-Kramer)

Soluble protein (% of crude protein, dry matter basis):

1. Control: 42.0%

2. Vitazyme on seed + 100% N: 46.3%

3. Vitazyme in fertilizer: 48.3%

4. Vitazyme on seed + leaves + 100% N: 35.3%

5. Vitazyme on seed + leaves + 70% N: 49.3%
LSD_0.1=6.9%

Trt. 5>Trt. 1 and 4; Trt. 3>Trt. 4 at P=0.10 (Tukey-Kramer)

Neutral detergent Insoluble* (% of dry matter):

1. Control: 1.77%

2. Vitazyme on seed + 100% N: 1.67%

3. Vitazyme in fertilizer: 1.57%

4. Vitazyme on seed + leaves + 100% N: 1.80%

5. Vitazyme on seed + leaves + 70% N: 1.77%
LSD_0.1=0.26%

*Utilizable protein that is not termed available

Starch (% of dry matter):

1. Control: 16.8%

2. Vitazyme on seed + 100% N: 16.6%

3. Vitazyme in fertilizer: 17.8%

4. Vitazyme on seed + leaves + 100% N: 17.9%

5. Vitazyme on seed + leaves + 70% N: 18.2%
LSD_0.1=2.0%

Digestible neutral detergent fiber (% of dry matter):

1. Control: 71.9%

2. Vitazyme on seed + 100% N: 69.1%

3. Vitazyme in fertilizer: 67.2%

4. Vitazyme on seed + leaves + 100% N: 70.7%

5. Vitazyme on seed + leaves + 70% N: 74.2%
LSD_0.1=4.1%

Trt. 5>Trt. 2 and 3; Trt. 1>Trt. 3 at P=0.10 (Tukey-Kramer)

In vitro net energy of lactation (Mcal/lb of dry matter):

1. Control: 0.743

2. Vitazyme on seed + 100% N: 0.720

3. Vitazyme in fertilizer: 0.717

4. Vitazyme on seed + leaves + 100% N: 0.737

5. Vitazyme on seed + leaves + 70% N: 0.763
LSD_0.1=0.021%

Trt. 5>Trt. 2 3, and 4; Trt. 1>Trt. 2 and 3 at P=0.10 (Tukey-Kramer)

Milk estimates from Milk 2000 calculations:

1. Control: 19,607 lb of milk/acre

2. Vitazyme on seed + 100% N: 21,075 lb of milk/acre (+7%)

3. Vitazyme in fertilizer: 21,075 lb of milk/acre (+9%)

4. Vitazyme on seed + leaves + 100% N: 20,749 lb of milk/acre (+5%)

5. Vitazyme on seed + leaves + 70% N: 21,883 lb of milk/acre (+11%)

Fertilization:  80 lb/acre N + 145 lb/acre K₂O plowed down in the fall; 80 lb/acre N topdressed July 15

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Arkport, New York

(Stutzman Research Farm)

Variety:  Agway 3311

Planting date:  June 7, 2002

Soil type:  silt loam

Seeding rate:  26,000 seeds/acre

Row spacing:  30 inches

Replicates:  five

Soil pH:  6.6

Harvest date:  September 20, 2002

Vitazyme application:  (1) 13 oz/acre dribbled over the dry fertilizer placed 2 in x 2 in below and beside the seeds, at planting for Treatments 2 and 3; (2) 13 oz/acre sprayed over the leaves and soil at the V6 stage of Treatments 2 and 3

Silage yield:

Control (no Vitazyme): 18.74 tons/acre
60% N + Vitazyme: 19.86 tons/acre (+6%)

100% N + Vitazyme: 20.56 tons/acre (+10%)
 

Crude protein (% of dry matter):

Control (no Vitazyme): 8.24% (a)
60% N + Vitazyme: 8.26% (a)

100% N + Vitazyme: 8.40% (a)

LSD_0.1=0.32

Neutral detergent fiber digestibility (% of dry matter):

1. Control (no Vitazyme): 57.3% (b)
2. 60% N + Vitazyme: 56.2% (b)

3. 100% N + Vitazyme: 60.7% (a)

LSD_0.1=1.7; P=0.0035

Trt. 3>Trt. 1 and 2 at P=0.10 (Tukey Kramer)

Milk per ton of silage*:

1. Control (no Vitazyme): 3,356.2 lb of milk/ton (b)
2. 60% N + Vitazyme: 3,330.7 lb of milk/ton (b)

3. 100% N + Vitazyme: 3,564.3 lb of milk/ton (a) (+6%)

LSD_0.1=69.0; P=0.0004

Trt. 3>Trt. 1 and 2 at P=0.10 (Tukey Kramer)

*This value is an index of how much milk would be produced from a ton of silage based on the quality of the silage.

Milk per acre*:

1. Control (no Vitazyme): 20,159 lb of milk/acre (b)
2. 60% N + Vitazyme: 21,078 lb of milk/acre (b) (+5%)

3. 100% N + Vitazyme: 23,472 lb of milk/acre (a) (+16%)

LSD_0.1=0.52; P=0.0003

Trt. 3>Trt. 1 and 2 at P=0.19 (Tukey Kramer)

*This figure is an index of (milk/ton of silage) (tons of silage) (tons of silage/acre) = milk/acre.

Milk income:

Control (no Vitazyme): $3,023.85/acre
60% N + Vitazyme: $3,161.70/acre (+$137.85/acre)

100% N + Vitazyme: $3,520.80/acre (+$496.95/acre)

Fertilization:  All areas received 125 lb/acre of a 10-30-10 starter, and no P or K.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Tipton, California

Variety:  Pioneer 33 J56 silage corn

Planting date:  June 25, 2003

Soil type:  clayey

Previous crop:  wheat for silage

Harvest date:  October 16, 2003

Vitazyme application:  Vitazyme was applied to Treatments 2,3, and 5, at 13 oz/acre each time, at planting on the seed row for Treatments 2 and 3, and also on the leaves 22 days after planting for Treatment 2, and without a seed application for Treatment 5.  A Tulare Ag Products mix (humates, organic acids, and amino acids) was also applied with the Vitazyme.

Leaf Chlorophyll:

Control: 52.5 SPAD units
Vitazyme: 59.5 SPAD units (+7.0 SPAD units)

Dry matter yield:

Control: 8.25 tons/acre
Vitazyme twice : 8.86 tons/acre (+7%)

Vitazyme at planting: 8.87 tons/acre (+7%)

Commercial fertilizer only: 8.65 tons/acre

Commercial fertilizer + Vitazyme: 8.53 tons/acre

Total digestible nutrient yield:

Control: 5.93 tons/acre
Vitazyme twice : 6.41 tons/acre (+8%)

Vitazyme at planting: 6.43 tons/acre (+8%)

Commercial fertilizer only: 6.19 tons/acre

Commercial fertilizer + Vitazyme: 6.15 tons/acre

Fertilization:  All areas received a "manure water" application before planting.  The commercial fertilizer application is not known.