| Vitazyme Field Trial Results: 1995-Present Corn |
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Miles, Texas |
Variety: Pioneer 3299 Planting date: Early May, 1996 Row spacing: 30 inches Population: 28,000 seeds/acre Harvest date: August 15, 1996 Irrigation: Center pivot, using San Angelo city wastewater |
Vitazyme application: Vitazyme applied at 16 oz/acre, along the seed row at planting |
Corn Yield (0.615-acre strips harvested):
Control: 92.8 bu/acre Corn Test Weight:
Control: 55 lb/bu
Income Increase: +$69.30/acre 
|
|
Fertilization: 95 lb/acre of 10-34-0 (%N-P2O5-K2O) starter fertilizer, plus some zinc (1 qt/acre); some nutrients from the sewage water |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Pella, Iowa |
Variety: Wilson 1581 Planting date: May 6, 1996 Soil type: silty clay loam Population: 25,800 seeds/acre Row spacing: 30 inches Harvest date: October 16, 1996 |
Vitazyme application: Vitazyme was sprayed on the soil at 13 oz/acre at planting (May 6), and again when the plants were 24 inches tall. |
Corn Yield:
Control: 194 bu/acre Income Increase: +$38.47/acre
Chlorophyll Content (August 9):
Control: 46.9 SPAD units
|
|
Fertilization: 130 lb/acre N and 80 lb/ace S were applied before planting as a dry fertilizer. Another 13 lb/acre of N was also applied at planting as a 28% N solution. |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Middle Point, Ohio
|
Variety: Pioneer 3394 Planting date: May 20, 1996 Soil type: silty clay Population: 27,000 seeds/acre Harvest date: November 13, 1996 |
Vitazyme application: Vitazyme at 13 oz/acre on the seeds at planting |
Moisture, at harvest:
Control: 14.9% Dry Matter, at harvest:
Control: 85.1%
Crude Protein, (dry):
Control: 9.2%
Acid Detergent Fiber:
Control: 3.5%
Total Digestible Nutrients:
Control: 88.0%
Ash:
Control: 1.46%
|
|
Fertilization: At planting, 5 gal/acre of 2-20-18 (%N-P2O5-K2O) + sugar + 1.7 pt/acre Zn + 10 pt/acre Mn applied directly on the seeds |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Leroy, New York |
Variety: DeKalb 446 Planting date: May 30, 1996 Population: 26,500 seeds/acre Harvest date: unknown |
Vitazyme application: Vitazyme was applied directly on the seeds at 12 oz/acre with the starter fertilizer. |
Corn Yield:
Control: 121 bu/acre
Corn quality, Vitazyme vs. the control: Phosphorus: +19% Magnesium: +57% Salts: +30% Total nutrient elements: +24% Acid detergent fiber: +22% Relative feed value: +83% Dry matter intake: +84% Nitrates: -11%
|
|
Fertilization: 5 gal/acre 10-20-10 starter fertilizer directly on the seeds. On July 15, 20 gal/acre liguid nitrogen (30% actual N) sidedressed. Later, 2 qt/acre liquid-Ca (8-0-0-10) plus herbicide (1 qt/acre Marksman) were applied. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Danville, Iowa |
Variety: Querna 7670 Planting date: June 5, 1996 Soil type: Mahaska silt loam Seeding depth: 2 inches Population: 26,000 seeds/acre Harvest date: unknown Row spacing: 30 inches |
Vitazyme application: Vitazyme was applied at 13 oz/acre in the seed row at planting, and again on July 11, on the leaves and soil when the plants were 18 inches high. |
Corn Yield (Low nitrogen):
Control: 127.6 bu/acre
Corn Yield (High nitrogen):
Control: 155.2 bu/acre
Income Increase (Low Nitrogen): +$82.80/acre
Income Increase (High Nitrogen): +$31.20/acre
|
|
Fertilization: Nitrogen was applied as urea (46% N) on June 3. For the 80 lb/acre rate 174 lb/acre was applied, and for the 120 lb/acre rate 261 lb/acre of urea was applied. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Cedar Falls, Iowa (ACRES Research Farm) |
Variety: CFS 6326 Planting date: May 21, 1996 Seeding depth: 1.5 inches Population: 29,900 seeds/acre Harvest date: unknown |
Vitazyme application: Vitazyme was applied at 13 oz/acre on the seeds at planting, and again on July 2, on the soil using drop nozzles, at the 7-leaf stage. |
Corn Yield (Low nitrogen):
Control: 154.1 bu/acre
Corn Yield (High nitrogen):
Control: 166.4 bu/acre [This slight decrease at high N levels illustrates the reduced Vitazyme response sometimes-but not always-experienced with very high N applications. In 2004, a very high yield year, at high N levels Vitazyme boosted yields by 14 to 20 bu/acre in replicated tests. The product usually works best when N levels are reduced from optimum levels.]
Income Increase (Low Nitrogen): +$32.70/acre
Income Decrease (High Nitrogen): -$6.60/acre
|
|
Fertilization: Nitrogen was applied as a 28% nitrogen solution on May 17. Two levels of N were used: 80 lb/acre, and 120 lb/acre. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1996 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: unknown Planting rate: thinned to three plants/pot Pot size: 1 gallon Trial duration: 33 days Replicates: 6 |
Vitazyme application: Vitazyme applied at planting and weekly at 100 ml of a 0.1% solution. |
Plant dry weight:
Control: 3.12 g/plant *Significantly greater than the control at P=0.05
|
|
Fertilization: moderate levels |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1997 |
Shortsville, New York |
Variety: Pioneer 3752 (97 day) Planting date: May 14, 1997 Row width: 30 inches Population: 30,000 seeds/acre Soil type: Ontario loam, 3 to 10% slope Harvest date: November 26, 1997 |
Vitazyme treatment: 12 oz/acre of Vitazyme, on the seeds at planting with the liquid fertilizer |
Yield Increase: Control: 129.6 bu/acre Vitazyme: 142.2 bu/acre (+10%)
Test Weight Increase: Control: 53 lb/bu Vitazyme: 55 lb/bu (+2 lb/bu)
Income Increase: +$63.40/acre
|
|
Fertilization: 10 tons/acre of manure, plus 26 gal/acre of 30% nitrogen and 4 gal/acre of ammonium thiosulfate over the entire area. The control area received an additional 8 gal/acre of 9-18-9 plus 4 gal/acre of 0-0-30 at planting. The Vitazyme treatment received 2.7 gal/acre of 9-18-9 plus 1.3 gal/acre of 0-0-30 at planting, 33% of the control treatment. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1997 |
Ames, Iowa (Iowa State University) |
Variety: Pioneer 3489 Planting date: May 6, 1997 Row width: 30 inches Population: 27,700 seeds/acre Harvest date: October 15, 1997 |
Vitazyme application: Vitazyme at 13 oz/acre on the seeds at planting, and/or a soil/foliar treatment at the V6 stage at 13 oz/acre |
Corn Yield:
Control: 148.9 bu/acre Vitazyme at V6: 156.0 (+5%) Vitazyme at planting and V6: 152.4 (+2%)
|
|
Fertilization: Nitrogen was applied before planting at 125 lb/acre. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1997 |
Ames, Iowa (Iowa State University) |
Variety: Pioneer 3489 Planting date: May 6, 1997 Row width: 30 inches Population: 27,700 seeds/acre Previous crop: soybeans Harvest date: October 15, 1997 |
Vitazyme application: Vitazyme on the seeds at planting at 13 oz/acre, and later to the soil and foliage at the V6 stage |
Corn Yield:
Control: 161.1 bu/acre
Income Increase: +$12.30/acre
|
|
Fertilization: Nitrogen was applied to all areas before planting at 100 lb/acre. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1997 |
Ames, Iowa (Iowa State University) |
Variety: Pioneer 3489 Seeding date: May 6, 1997 Row width: 30 inches Population: 27,700 seeds/acre Previous crop: soybeans Harvest date: October 15, 1997 |
Vitazyme application: Three applications of Vitazyme, each at 13 oz/acre: at planting, at one leaf, and at six leaves (a total of 30 oz/acre) |
Corn Yield:
Control (0 lb N/acre): 151.7 bu/acre
Control (50 lb N/acre): 183.5 bu/acre
Control (100 lb N/acre): 191.3 bu/acre [Note the greater yield improvement at the lowest N levels.]
Income Increase (50 lb N/acre): +$21.30/acre
|
|
Fertilization: Nitrogen was applied at 0 lb/acre, 50 lb/acre, or 100 lb/acre, depending on the plot, before planting. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1997 |
Bagley, Iowa |
Variety: De Kalb SR corn Seeding date: May 23, 1997 Row width: 30 inches Row depth: 2 inches deep Population: 25,700 seeds/acre |
For both treatments, Vitazyme was applied to the seed row at 13 oz/acre, and also on the foliage and soil at the V-10 stage at 13 oz/acre. |
Decrease in grain moisture (80 lb N/acre): Control:17.77% Vitazyme: 16.97% (0.8%) [Note: Due to an exceptionally good year no significant yield differences were detected, but grain moisture was reduced significantly.]
|
|
Nitrogen was applied at 80 lb/acre or 120 lb/acre to the appropriate plots before planting. |
Non-Replicated Study (Organic)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1998 |
Garretson, South Dakota |
Variety: DeKalb DK483 (100-day) Seeding rate: 18,500 seeds/acre (36-inch rows) Seeding date: May 7, 1998 Harvest date: November 30, 1998 to December 4, 1998 |
Vitazyme application: Vitazyme 13 oz/acre was applied at planting, half in the band above the seed row and half in the band below the seeds |
Leaf Chlorophyll:
Control: 56.2 SPAD units
Grain test weight: The Vitazyme treated corn yielded grain weighing 60 lb/bu.
[Note: No yield comparison was made in this study.]
|
|
Fertilization: At planting, 3 gallons per acre of liquid fish, an herbal blend, and 2 oz/acre of Nutrapathic Soil Conditioner were applied in an 8-inch band on the soil above the seeds, and below the seed row in a strip. Ten gallons of solution per acre were used in each band. |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1998 |
Seneca, Kansas |
Variety: Hoegemeyer (110 day) Population: 19,500 plants/acre Planting date: unknown Soil: clayey Previous crop: soybeans Herbicide: post-emergent application Harvest date: October 20, 1998 |
Vitazyme application: Vitazyme 13 oz/acre was applied with the starter fertilizer directly on the seeds at planting. The control area received Gro-Zyme biostimulant in the starter fertilizer. |
Corn Yield:
Gro-Zyme (80 lb N/acre): 131.96 bu/acre
Income Increase: +$6.74/acre
|
|
Fertilization: All areas received 30 gal/acre of 28% N preplant, as well as 500 lb/acre of high-calcium lime (96% CaCO3) in the spring. Starter (5-20-5% N-P2O5-K2O) was also applied over all areas directly on the seeds at planting at 5 gal/acre. All areas had received Gro-Zyme Biostimulant for the previous 20 years. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1998 |
Malden, Missouri (Aycock Agricultural Services) |
Variety: Garst 8300 Planting rate: 23,600 plants/acre Planting date: April 4, 1998 Previous crop: cotton Soil type: sandy loam Irrigation: all plots were furrow irrigated Harvest date: September 5, 1998 |
Vitazyme application: Vitazyme 13 oz/acre, sprayed on the leaves and soil at the four-leaf stage |
Corn Yield:
Control: 102.0 bu/acre
Leaf Chlorophyll:
Control: 39.1 SPAD units
Income Increase: +$30.87/acre
|
|
Fertilization: All areas received 175-40-40 lb/acre of N-P2O5-K2O. Ninety lb/acre of N was applied preplant, and 85 lb/acre sidedressed 40 days after planting |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1999 |
Geneseo, New York |
Variety: unknown Planting date: unknown Harvest date: November 10, 1999 |
Vitazyme application: Vitazyme 13 oz/acre at planting |
Corn Yield:
Control: 182.6 bu/acre
Leaf Chlorophyll:
Control: 54.7 SPAD units
Income Increase: +$22.50/acre
|
|
Fertilization: unknown, but the same over all areas |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1999 |
Shortsville, New York |
Variety: Pioneer 3573 Planting date: May 4, 1999 Seeding rate: 30,000 seeds/acre planted; 28,000 plants/acre final Weather conditions: very dry season-long |
Vitazyme application: Vitazyme at 13 oz/acre mixed with the 3-18-18, on the seeds at planting |
Corn Yield (50% fertilizer):
Control: 76.12 bu/acre
Test weight increase: + 0.5 lb/bu
Income Increase: +$17.67/acre
|
|
Fertilization: All areas received a broadcast application of 10 lb/acre (NH4)2SO4 (21% N) + Boron + Copper, 500 lb/acre EnviroSoil (composted sewage sludge mixed with high-calcium lime), 32 gal/acre 30% UAN, and 1 gal/acre liquid C-nitrate. At planting the control received 10 gal/acre of 3-18-18 while the Vitazyme treated area received 5 gal/acre of 3-18-18. Total N per acre for the broadcast fertilizer was 107 lb/acre, excluding the compost, for the control, and 106 lb/acre for the Vitazyme treatment. |
Non-Replicated Study - Organic
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
1999 |
Olivia, Minnesota |
Variety: Enestvedts 670 Planting date: April 24, 1999 Maturity: 102 days Population: 31,700 seeds/acre Row spacing: 30 inches Previous crop: soybeans Harvest date: October 19, 1999 Weather: very cold and wet in May |
Vitazyme application: Vitazyme at 13 oz/acre mixed with molasses, on the seeds at planting. |
Corn Yield:
Control: 150.0 bu/acre [Note: The weather was very cold and wet early in the season, which reduced product effectiveness for and early application.]
Income Increase: +$6.76/acre
|
|
Fertilization: All areas received 3 tons/acre of poultry litter in March. At planting 2 gallons/acre of molasses were applied to the seed row. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Ames, Iowa (Iowa State University of Science and Technology) |
Variety: Pioneer 3589 Population: 30,000 seeds/acre Soil type: Clarion clay loam Planting date: April 21, 2000 Previous crop: corn Row spacing: 30 inches Depth of Planting: 1.5 inches Harvest date: unknown |
Vitazyme application: (1) seed-row application at 13 oz/acre at planting; (2) soil/foliar application at 13 oz/acre on June 18 at the 8-leaf stage, using 50 gallon/acre of solution |
Corn Yield (100 lb N):
Control 1: 88.8 bu/acre Control 2: 91.6 bu/acre *Significantly greater than the control at P=0.10 (Tukey's Test).
Corn Yield (150 lb N):
Control 1: 103.6 bu/acre Control 2: 104.3 bu/acre
[Note: A Japanese product called MC, a growth regulator extracted from fruit tree prunings, did not increase corn yield or test weight in this study.]
|
|
Fertilization: Phosphorus and potassium were maintained above medium soil test levels. Nitrogen was applied preplant incorporated at 100 and 150 lb/acre of actual N (as urea, 45% N) to the appropriate treatments. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Cedar Falls, Iowa(Agricultural Custom Research and Education Services) |
Variety: Pioneer 34G130 Planting date: April 28, 2000 Seeding rate: 29,900 seeds/acre Previous crop: soybeans Soil type: Maxfield silty clay loam (2.8% organic matter, pH 6.8) Row spacing: 30 inches Harvest date: September 25, 2000 |
Vitazyme application: (1) Vitazyme at 13 oz/acre on the seed at planting; (2) Vitazyme at 13 oz/acre at knee height on the leaves and soil |
Corn Yield:
Control: 115.6 bu/acre (a) Means followed by the same letter are not significantly different at P=0.11 (Duncan's Test).
Grain Yield per Plant:
Control: 0.512 lb/plant (a) Means followed by the same letter are not significantly different at P=0.15 (Duncan's Test). [Note: A humate product called Actagro provided a yield increase equal to Vitazyme, but produced significantly less grain/plant.]
Income Increase: +$13.65/acre
|
|
Fertilization: 160 lb/acre of nitrogen as anhydrous-NH3; no phosphorous and potassium |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Arkport, New York (Stutzman Research Farm) |
Variety: Golden Harvest h7615 Roundup Ready Planting date: May 26, 2000 Seeding rate: 30,000 seeds/acre Soil type: silt loam Row spacing: 30 inches Harvest date: November 11, 2000 |
Vitazyme application: Vitazyme at 6 oz/acre on the seeds at planting (Treatment 2); 6 oz/acre sprayed on the leaves and soil with the herbicide |
Corn Yield:
Control: 151 bu/acre (a) Vitazyme foliar: 141 bu/acre (a) *Means followed by the same letter are not significantly different at P=0.10 (Tukey). [No significant yield differences]
|
|
Fertilization: 175 lb/acre N and 120 lb/acre K2O preplant incorporated and sidedressed, plus 100 lb/acre 5-24-25% N-P2O5-K2O + micronutrients starter at planting |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Iju/itaogbolu, Akure North Local Government Area, Ondo State, Nigeria (Department of Agriculture, Ondo State, Nigeria) |
Variety: unknown Planting date: late season of 2000 Soil type: unknown Row spacing: unknown Harvest date: unknown Reps: three |
Vitazyme application: Treatments 1, 2, and 3 received a 5% Vitazyme spray on the corn seeds before planting, and the newly emerged plants and soil received 1 liter/ha (13 oz/acre) two weeks after planting. |
Plant height: Control: 1.2 m
Vitazyme only: 1.5 m
(+25%) 200 NPK + Vitazyme: 1.6 m (+33%) 100 NPK: 1.6 m (+33%) 200 NPK: 1.6 m (+33%)
Ears per square meter: Control: 2.3
Vitazyme only: 4.3
(+17%) 200 NPK + Vitazyme: 4.0(+104%) 100 NPK: 4.3 (+74%) 200 NPK: 2.3 (+87%)
Ear length: Control: 8.6 cm
Vitazyme only: 9.3 cm
(+8%) 200 NPK + Vitazyme: 15.0 cm (+74%) 100 NPK: 14.4 cm(+67%) 200 NPK: 15.1 cm (+76%)
Ear weight: Control: 60.0 grams
Vitazyme only: 82.3 grams
(+37%) 200 NPK + Vitazyme: 110.0 grams(+83%) 100 NPK: 89.3 grams (+49%) 200 NPK: 107.0 grams (+78%)
Grain yield: Control: 83.0 g/m2
Vitazyme only: 196.2 g/m2
(+136%) 200 NPK + Vitazyme: 345.3 g/m2(+316%) 100 NPK: 255.0 g/m2 (+207%) 200 NPK: 328.3 g/m2 (+296%)
|
|
Fertilization: Treatments 2 and 4 received 100 kg/ha of an unknown fertilizer formulation two weeks after planting, while Treatments 3 and 5 received 200 kg/ha of this fertilizer two weeks after planting. |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Waterloo, New York |
Variety: Agway 501 Planting date: May 31, 2000 Seeding rate: unknown Row spacing: 30 inches Harvest date: December 3, 2000 |
Vitazyme application: Vitazyme at 13 oz/acre with the herbicide, at 18-inches corn height |
Corn Yield:
Control: 141 bu/acre
Income Increase: +$24.50/acre
|
|
Fertilization: 380 lb/acre of a urea and potash mixture, broadcast before planting and incorporated; 250 lb/acre of 11-37-0% N-P2O5-K2O as a starter |
Replicated Study (Humate Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Mexican flinty yellow dent Planting date: March 17, 2000 Planting rate: seven seeds per pot, thinned to three plants per pot Pot size: 1 gallon Humic acid material: Actagro "Liquid Humus", Biola, California Watering: as needed Harvest date: April 17, 2000 |
Vitazyme application: Vitazyme at 13 oz/acre Liquid humus application: 4 gal/acre |
Plant height*: Control: 43.8 cm (b) Liquid humus: 43.3 cm (b) (-1%)
Vitazyme: 50.4 cm (a)
(+15%) *Means followed by the same letter are not significantly different at P=0.10. LSD0.10 = 5.1.
Plant dry weight*: Control: 3.54 grams (c) Liquid humus: 3.85 grams (b) (+9%)
Vitazyme: 4.19 grams (a)
(+18%) *Means followed by the same letter are not significantly different at P=0.10. LSD0.10 = 0.63
|
|
Fertilization: none |
Replicated Study (Fish and MC Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Mexican white dent Soil type: "Cherry Lane" fine sandy loam Replications: five Pot size: 1 gallon |
Vitazyme application: 100 ml of a 0.01% solution after planting Fish application: 100 ml of a 0.01% solution after planting MC Liquid application: 100 ml of a 0.01% solution after planting |
Plant height*: Control: 41.1 cm (c) Fish: 42.3 cm (bc) (+3%) Vitazyme: 46.3 cm (ab) (+13%) MC Liquid: 46.6 cm (a) (+13%) Vitazyme + Fish: 50.1 cm (a) (+22%) *Means followed by the same letter are not significantly different at P=0.10.
Plant dry weight*: Control: 3.92 grams (c) Fish: 3.95 grams (c) (+1%) Vitazyme: 4.27 grams (bc) (+8%) MC Liquid: 5.40 grams (a) (+38%) Vitazyme + Fish: 5.65 grams (a) (+44%) *Means followed by the same letter are not significantly different at P=0.10.
|
|
Fertilization: none |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Cedar Falls, Iowa |
Variety: Pioneer 33A14 Soil type: Maxfield silt loam, surface soil Tray size and type: 10 x 21 inches, slotted tray inside a solid tray, and a paper liner below
|
Vitazyme application: The equivalent of 13 oz/acre Vitazyme was applied to the soil surface at planting. |
Water retention, tray weight less 6000 grams (7 days after planting)*: Control: 11.8 grams Vitazyme: 57.8 grams *There were no significant differences between the treatments for plants emerged, plant height, or plant vigor for the 13 day period.
Water retention, tray weight less 6000 grams (13 days after planting)*: Control: -168.3 grams Vitazyme: -116.3 grams *There were no significant differences between the treatments for plants emerged, plant height, or plant vigor for the 13 day period.
|
|
Fertilization: none |
Replicated Study (Fish Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Mexican flinty field corn Soil type: very fine sandy loam Replications: five Planting date: January 17, 2000 Planting date: 7 seeds/pot, thinned to 3 plants/pot |
Vitazyme application: Eight treatments were used. The fish was an enzymatically degraded product. For Vitazyme alone the 13 oz/acre rate was used, and for fish alone the 13 oz/acre rate was used. Combinations of the two always totaled 13 oz/acre of the combined products. Enzymatically degraded fish from Jedwards International in New Jersey was used in this study. |
Dry weight per pot*: 1 part Vitazyme, 9 parts fish: 42.5 grams (a) 9 parts Vitazyme, 1 part fish: 41.9 grams (a) 0 parts Vitazyme, 1 part fish: 41.7 grams (a) 1 part Vitazyme, 4 parts fish: 41.6 grams (a) 1 part Vitazyme, 1 part fish: 41.4 grams (a) 1 part Vitazyme, 0 parts fish: 40.7 grams (a) 0 parts Vitazyme, 0 parts fish: 40.4 grams (a) 4 parts Vitazyme, 1 part fish: 39.8 grams (a) *Means followed by the same letter are not significantly different at P=0.10.
|
|
Fertilization: none |
Replicated Study (Fish and MC Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2000 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Mexican flinty field corn Soil type: very fine sandy loam Replications: five Planting date: January 17, 2000 Planting date: 7 seeds/pot, thinned to 3 plants/pot |
Vitazyme application: Eleven treatments were used. For Vitazyme alone the 13 oz/ha was used, and for MC alone the 13 oz/acre rate was used. Combinations of the two or three products always totaled 13 oz/acre of the combined products. MC Liquid is a carbonaceous liquid derived from the burning of fruit tree prunings. Enzymatically degraded fish from Jedwards International in New Jersey was used in this study. |
Dry weight per pot*: 1 part Vitazyme, 0 parts MC, 0 parts fish: 4.60 grams (a) 8 parts Vitazyme, 1 part MC, 1 part fish: 4.58 grams (a) 1 part Vitazyme, 9 parts MC, 0 parts fish: 4.43 grams (ab) 9 parts Vitazyme, 1 part MC, 0 parts fish: 4.41 grams (ab) 0 parts Vitazyme, 1 part MC, 0 parts fish: 4.41 grams (ab) 40 parts Vitazyme, 1 part MC, 9 parts fish: 4.26 grams (ab) 1 part Vitazyme, 4 parts MC, 0 parts fish: 4.19 grams (ab) 49 parts Vitazyme, 1 part MC, 0 parts fish: 4.13 grams (ab) 4 parts Vitazyme, 1 part MC, 0 parts fish: 3.99 grams (ab) 1 part Vitazyme, 1 part MC, 0 parts fish: 3.83 grams (b) 0 parts Vitazyme, 0 parts MC, 0 parts fish: 3.75 grams (b) *Means followed by the same letter are not significantly different at P=0.10.
|
|
Fertilization: none |
Replicated Study (Product Synergisms)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Mexican flint corn Soil type: Bowie very fine sandy loam (50%) and Carl Pool Topsoil mix (50%) Replications: eight Pot size: 1 gallon Planting rate: seven seeds/pot, thinned to three plants/pot |
Biostimulant treatments: All products were applied at 100 ml of the solution per pot. Rates were those recommended by the manufacturer, except Vitazyme and H2O2 were applied at varied rates. Rates: Vitazyme, 0.1% Vitazyme, 0.01% H2O2, 40% H2O2, 1% H2O2, 0.01% Percplus, 0.01% Botanicare Blastof, 0.4% HB-101, 5 drops/gal Experimental-1, 0.01% Experimental-2, 0.01% Vinegar, 1%
|
Plant height*: H2O2, 40%: 87.6 cm (a) (+7%) Vitazyme, 0.1%: 85.2 cm (ab) (+4%) Vinegar: 84.3 cm (ab) (+3%) Vitazyme, 0.01%: 84.2 cm (ab) (+3%) Exp-2: 82.7 cm (ab) (+1%) H2O2, 0.01%: 82.1 cm (a) (+0%) Control: 82.1cm (ab) HB-101: 81.1 cm (ab) (-1%) Bot-Blast: 80.7 cm (ab) (-2%) H2O2, 1%: 80.5 cm (ab) (-2%) Exp-1: 80.2 cm (ab) (-2%) Percplus: 78.2 cm (ab) (-5%) *Means followed by the same letter are not significantly different at P=0.10 according to the Tukey-Kramer test.
Plant dry weight*: H2O2, 40%: 15.91 grams (a) (+22%) Exp-2: 13.92 grams (b) (+7%) Vitazyme, 0.1%: 13.81 grams (b) (+6%) Vitazyme, 0.01%: 13.60 grams (bc) (+5%) Vinegar: 13.22 grams (bcd) (+2%) Control: 12.99 grams (bcde) H2O2, 0.01%: 12.59 grams (bcde) (-3%) Bot-Blast: 12.17 grams (bcde) (-6%) HB-101: 12.10 grams (bcde) (-7%) Exp-1: 11.81 grams (cde) (-9%) H2O2, 1%: 11.69 grams (de) (-10%) Percplus: 11.32 grams (e) (-13%) *Means followed by the same letter are not significantly different at P=0.10 according to the Tukey-Kramer test.
|
|
Fertilization: none |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Cecilia, Kentucky |
Variety: Novartis 6367 Planting date: April 10, 2001 Seeding rate: 26,600 seeds/acre Soil type: unknown Row spacing: 30 inches Harvest date: October 11, 2001 |
Vitazyme application: Vitazyme at 13 oz/acre along with the 28% nitrogen solution, applied June 9 |
Corn Yield:
Control: 102.6 bu/acre Sidedress N, low: 124.4 bu/acre (+21%) Sidedress N, high: 138.1 bu/acre (+35%) Sidedress N, high + Vitazyme: 151.9 bu/acre (+48%)
Increased income for sidedressed Vitazyme + high N: $98.60
Return per dollar invested in Vitazyme: $9.95
|
|
Fertilization: 50-60-60 lb/acre N-P2O5-K2O preplant incorporated; sidedress fertilizer applied to appropriate areas |
Replicated Study (Bacteria and Restore Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Cedar Falls, Iowa (ACRES Research Farm) |
Variety: Pioneer 33P67 Planting date: May 9, 2001 Seeding rate: 29,900 seeds/acre Soil type: Kenyon loam Row spacing: 30 inches Planting depth: 1.5 inches Previous crop: soybeans |
Vitazyme application: 13 oz/acre in-furrow at planting (May 9) for the IF applications; 13 oz/acre over the soil (one week after planting May 16) for the BC application. Restore application: 2 oz/acre in-furrow at planting (May 9) for the IF application; 2 oz/acre over the soil (a week after planting on May 16) for the BC application. Bacteria application: 2 oz/acre in-furrow at planting (May 9) for the IF application; 2 oz/acre over the soil (a week after planting on May 16) for the BC application. |
Effects of Restore and Bacteria with Vitazyme at 120 lb/acre N Corn yield (in-furrow):
Vitazyme IF: 150.0 bu/acre Vitazyme IF + Restore IF: 156.3 bu/acre (+6.3 bu/acre) Vitazyme IF + Restore IF + Bacteria IF: 158.6 bu/acre (+8.6 bu/acre)
Corn yield (broadcast):
Restore BC + Bacteria BC:
150.5 bu/acre Vitazyme BC + Restore IF + Bacteria IF: 164.2 bu/acre (+13.7 bu/acre)
Effects of Restore with Vitazyme at 160 lb/acre N
Corn
yield (in-furrow): Vitazyme IF + Restore IF: 156.2 bu/acre (+3.2 bu/acre)
|
|
Fertilization: The starter (6-18-6% N-P2O5-K2O) on all plots was applied with the seeds at planting, and the nitrogen was applied pre-plant as a 28% nitrogen solution. |
Replicated Study (T-22 Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Spanish Fork, Utah (Brigham Young University Research Farm) |
Variety: B54v (Bird Hybrids) Planting date: April 30, 2001 Planting rate: thinned to 30,000 plants/acre Soil type: silty clay loam Row spacing: 36 inches between rows, 4 to 6 inches in rows Harvest date: September 27, 2001 |
Vitazyme application: For Treatment 6, 5 oz/acre were applied with the Plant Shield on the seeds at planting; for Treatment 7, the same was applied at planting as for Treatment 6 plus 13 oz/acre on the leaves and soil at 8 to 10 leaf stage (June 19; 18-inch height).
T-22 application: Tricoderma harzianum, strain T-22 [Plant Shield], was applied on the seeds at planting at 6 oz/acre. |
Corn height*: T-22 SP (1 oz): 66.3 inches (+4%) T-22 SP (2 oz): 65.2 inches (+3%) T-22 SP + Vit (1x): 65.0 inches (+2%)
T-22 SP (3 oz): 63.6 inches
(+0%) T-22 SP + Vit (2x): 61.3 inches (-4%) T-22 PB (6 oz): 59.8 inches (-6%) *SP = soluble powder; PB = planter box granules
Plants per 45 ft2*: Control: 33.50 T-22 SP + Vit (2x): 31.75 (-5%) T-22 PB (6 oz): 31.50 (-6%) T-22 SP + Vit (1x): 30.75 (-8%) T-22 SP (2 oz): 30.25 (-10%) T-22 SP (1 oz): 29.00 (-13%) T-22 SP (3 oz): 28.75 (-14%) *SP = soluble powder; PB = planter box granules
Ears per 45 ft2*: Control: 32.00 T-22 SP + Vit (1x): 30.25 (-5%) T-22 PB (6 oz): 30.25 (-5%) T-22 SP + Vit (2x): 29.25 (-9%) T-22 SP (2 oz): 28.50 (-11%) T-22 SP (3 oz): 27.00 (-16%) T-22 SP (1 oz): 25.75 (-20%) *SP = soluble powder; PB = planter box granules
Grain yield*: T-22 SP + Vit (2x): 249.7 bu/acre (+13%) T-22 SP (3 oz): 245.2 bu/acre (+11%) T-22 PB (6 oz): 226.8 bu/acre (+2%) Control: 221.9 bu/acre T-22 SP + Vit (1x): 214.7 bu/acre (-3%) T-22 SP (1 oz): 210.8 bu/acre (-5%) T-22 SP (2 oz): 188.4 bu/acre (-15%) *SP = soluble powder; PB = planter box granules Yield adjusted to 30,000 plants/acre at 15.5% moisture
Weight for 100 seeds*: T-22 SP (3 oz): 50.75 grams (+9%) T-22 SP + Vit (2x): 48.05 grams (+4%) T-22 PB (6 oz): 47.63 grams (+3%) T-22 SP (1 oz): 46.95 grams (+1%) Control: 46.33 grams T-22 SP + Vit (1x): 44.98 grams (-3%) T-22 SP (2 oz): 40.68 grams (-12%) *SP = soluble powder; PB = planter box granules Weight adjusted to 15.5% moisture
|
|
Fertilization: All plots received 93 lb/acre of N as urea (34-0-0) before planting. Another 53 lb/acre of N were applied on June 19. |
Testimonial
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Norfolk, Nebraska |
Variety: proprietary Planting date: unknown Seeding rate: unknown Row spacing: 30 inches Harvest date: December 3, 2000 Irrigation: center pivot |
Vitazyme application: (1) 13 oz/acre with the herbicide at planting (2) 13 oz/acre on the leaves and soil sometime later |
Field 1 Leaf Chlorophyll:
Control: 53.8 SPAD units
Comments:
Field 2 Leaf Chlorophyll:
Control: 53.8 SPAD units
Comments:
|
|
Fertilization: proprietary |
Testimonial
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Delavan, Minnesota |
Variety: unknown Soil type: Clarion-Nicollet-Webster series |
Vitazyme application: 13 oz/acre on the seeds at planting |
Leaf chlorophyll*:
Control: 35.8 SPAD units *Twenty leaves per treatment were examined
Comments noted on July 30, 2001: taller plants, larger stalks, darker green color (more chlorophyll), larger roots with more hair roots), and many more fine root hairs (noticed as disking was performed: roots came up as "bushy root balls").
|
|
Fertilization: unknown |
Replicated Study (T-22 Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Pencil cob corn Planting date: February 22, 2001 Planting rate: seven seeds/pot, thinned to three plants/pot Soil type: Bowie fine sandy loam Pot size: one gallon Replicates: five Trial termination: April 4, 2001 |
T-22 and Vitazyme application: T-22 was applied as a 1% Plant Shield suspension (1 oz/gallon of water), at 100 ml/pot at planting (soil), or as a spray on all leaf surfaces at three weeks after planting, along with 2 tbsp/gal of Sunspray crop oil. Vitazyme was applied with a 0.1 % solution (1 ml/liter) at 100 ml/pot at planting (soil), or as a 1% solution at 3 weeks after planting along with 2 tbsp/gal of Sunspray. |
Plant dry weight:
T-22, soil: 4.91 grams (a)
(+23%) Vitazyme, leaves: 4.84 grams (a) (+22%) Vitazyme, soil: 4.65 grams (ab) (+17%) T-22, leaves: 4.64 grams (ab) (+17%) T-22 + Vitazyme, soil: 4.58 grams (ab) (+15%) Control: 3.98 grams (b) *Means followed by the same letter are not significantly different at P=0.10 according to Duncan's Multiple Range Test. LSD0.10=0.44g.
|
|
Fertilization: none |
Replicated Study (Greenup Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: yellow dent Planting date: October 24, 2001 Planting rate: 7 seeds/pot, thinned to 3/pot Soil type: Bowie very fine sandy loam Pot size: one gallon Replicates: eight Trial termination: December 4, 2001 |
Vitazyme application: After planting on October 24, 100 ml of a 0.002% Vitazyme solution was applied to the soil surface of each pot for Treatment 2. This application was equal to the amount of Vitazyme contained in the Greenup of Treatment 4. Greenup - 6 + 16 application: Regular Greenup granules, a "Sucrate", were applied to the soil surface of the pots of Treatment 3 at 1 gram per pot; this rate equaled 10 lb/1,000 ft2. The Greenup for Treatment 4 had been prepared earlier at the facilities of American Minerals. Two ounces of Vitazyme were mixed with the binder of 50 lb of Greenup during processing, a 0.04 oz/lb rate. At 10 lb/1,000 ft2 of Greenup application, this would then give a Vitazyme application rate of about 18 oz/acre. This product was also applied at 1 gram per pot, as for Treatment 3. |
Plant height*:
Control: 75.2 cm (a) Vitazyme + Greenup: 72.0 (a) (-4%) Vitazyme: 71.9 (a) (-4%) *Means followed by the same letter are not significantly different at P=0.10, according to Student-Newman-Keuls Test. LSD0.10=4.1 cm.
Plant dry weight*:
Vitazyme + Greenup: 5.26 grams
(a)
(+30%) Vitazyme: 4.52 grams (a) (+11%) Control: 4.00 grams (a) *Means followed by the same letter are not significantly different at P=0.10, according to Student-Newman-Keuls Test. LSD0.10=0.52 grams.
|
|
Fertilization: none |
Replicated Study (T-22 Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Ames, Iowa (Iowa State University of Science and Technology) |
Variety: P-34G13 Planting date: May 16, 2001 Seeding rate: 30,000 seeds/acre Depth of planting: 1.5 inches Previous crop: corn Row spacing: 30 inches Harvest date: unknown |
Vitazyme application: Treatments 2, 3, 6, and 7 received 13 oz/acre in the seed row at planting, while Treatments 3 and 7 received an additional 13 oz/acre on the soil and forage on July 11. Vitazyme application: T-22 is Trichoderma harzianum, strain T-22. The soluble powder form was applied in the seed row at 1 oz/acre for Treatments 4, 5, 6, and 7, and again at 1 oz/acre over the leaves and soil for Treatments 5 and 7 on July 11. |
Plot Area 1: Vitazyme only: Corn Yield*:
Control: 144.3 bu/acre (b) Vitazyme on seeds: 153.0 bu/acre (a) (+6%) *Means followed by the same letter are not significantly different at P=0.10 according to the Tukey-Kramer Test. LSD0.10 = 7.4 bu/acre.
Plot Area 2: T-22 only: Corn Yield*:
Control: 149.2 bu/acre (a) T-22 on seeds: 151.3 bu/acre (a) (+1%) *Means followed by the same letter are not significantly different at P=0.10 according to the Tukey-Kramer Test. LSD0.10 = 8.2 bu/acre.
Plot Area 3: Vitazyme + T-22: Corn Yield*:
Control: 144.3 bu/acre (a) Vitazyme + T-22 on seeds: 148.4 bu/acre (a) (+3%) *Means followed by the same letter are not significantly different at P=0.10 according to the Tukey-Kramer Test. LSD0.10 = 7.4 bu/acre.
|
|
Fertilization: Phosphorus and potassium levels were maintained above medium soil test levels. Nitrogen was applied preplant incorporated at 100 lb N/acre |
Non-Replicated Study (Restore and Soil Conditioner Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2001 |
Albion, New York |
Field 22 Variety: Pioneer 38PO5 Planting date: May 10, 2001 Seeding rate: 32,000 seeds/acre Previous crop: wheat Field size: 54 acres Row width: 30 inches Harvest date: October 17 or 18
Field 529 Variety: NK3030BT Planting date: May 7, 2001 Seeding rate: 32,000 seeds/acre Previous crop: wheat Field size: 82 acres Row width: 30 inches Harvest date: from October 21 to 28
Field 510 Variety: Pioneer 38PO5 Planting date: May 15, 2001 Seeding rate: 32,000 seeds/acre Previous crop: wheat Field size: 75 acres Row width: 30 inches Harvest date: October 27 or 28 |
Vitazyme application: 13 oz/acre with the liquid N, preplant incorporated Ca-nitrate, SC, and Restore application: applied at recommended rates, with the liquid N and Vitazyme preplant incorporated |
Field 22 Corn Yield:
Control: 92.6 bu/acre
Field 529 Corn Yield:
Control: 137.5 bu/acre Vitazyme + SC, and Restore: 147.1 bu/acre (+7%)
Field 510 Corn Yield:
Control: 125.1 bu/acre Vitazyme + Ca: 141.1 bu/acre (+13%)
|
|
Fertilization: 125 lb/acre of liquid N (32%) preplant incorporated; 200 lb/acre of 11-22-22% N-P2O5-K2O + Zn and B, at planting. |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2002 |
Blue Grass, Iowa |
Variety: LG 2637 Planting date: May 16, 2002 Seeding rate: 30,000 seeds/acre Soil type: Fayette clay loam Row spacing: 30 inches Previous crop: soybeans Soil fertility: low: 110 ppm K, organic matter 1.5%, pH 6.9 Harvest date: November 1, 2002 |
Vitazyme application: Vitazyme at 12 oz/acre with the herbicide |
Corn Yield:
Control: 198 bu/acre Grozyme: 200 bu/acre (+1%)
Corn Test Weight:
Control: 56 lb/bu Grozyme: 56 lb/bu (0 lb/bu)
Income Increase with Vitazyme: +$15.00/acre
|
|
Fertilization: 125-0-0-12.5 % N-P2O5-K2O-S at planting, plus 5 gal/acre of Ag Spectrum "Clean Start" (Grozyme) in the seed furrow |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2002 |
Cedar Falls, Iowa (Agricultural Custom Research and Education Services; ACRES) |
Variety: Kruger 9912 RR Planting date: May 7, 2002 Seeding rate: 27,000 seeds/acre Soil type: Maxfield silty clay loam Row spacing: 30 inches Previous crop: soybeans Planting depth: 1.5 inches |
Vitazyme/Roundup application: 20 oz/acre on the seeds at planting Roundup herbicide (Glyphosate) was applied at 100% and 25% of normal rates, with or without Vitazyme, using four replicates. |
Weed control on June 20, July 3, and August 9 for Giant Foxtail, Smooth Pigweed, Dandelion, Velvetleaf, Giant Ragweed, Common Lambsquarters, Pennsylvania Smartweed, and Prickly Lettuce: Vitazyme did not significantly change the weed kill from Roundup for any weed species at either application level by August 9.
Weed control for all weeds combined, % control*:
Roundup, 100% rate: 90.5% (a)
Roundup, 25% rate: 77.5% (b) *Means followed by the same letter are not significantly different at P=0.10. Vitazyme did not significantly change the weed kill from Roundup at either application level.
Grain yield*:
Roundup, 100% rate: 180.2 bu/acre
(a)
Roundup, 25% rate: 162.5 bu/acre
(a) *Means followed by the same letter are not significantly different at P=0.10.
Grain per plant*:
Roundup, 100% rate: 0.38
lb/plant (a)
Roundup, 25% rate: 0.38
lb/plant (a) *Means followed by the same letter are not significantly different at P=0.10.
Grain income*:
Roundup, 100% rate:
$468.52/acre
Roundup, 25% rate:
$422.50/acre *Means followed by the same letter are not significantly different at P=0.10.
|
|
Fertilization:
160 lb/acre N as 28-0-0 (UAN) over all areas, preplant incorporated |
Replicated Study (Awaken Synergism)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2002 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: yellow dent Planting date: November 21, 2001 Planting rate: 7 seeds/pot, thinned to 3/pot Soil type: Bowie very fine sandy loam Pot type: 1 gallon Trial termination: January 8, 2002 |
Vitazyme application: After planting, 100 ml of a 0.01% Vitazyme solution was applied to the soil surface of each pot for Treatment 2. This rate is higher than recommended, but used so as to obtain a 50-50 mixture of the two products. Awaken application: Awaken was applied to the soil surface of the pots of Treatment 3 as 100 ml of a 0.01% solution; this is equivalent to 71 oz/acre, the recommended rate for this experiment. The Awaken for Treatment 4 was mixed at the same percentage with 0.01% Vitazyme, which was also applied at 100 ml/pot. |
Plant height*:
Control: 34.3 cm (c) Awaken: 43.4 cm (ab) (+27%) Vitazyme + Awaken: 45.4 cm (a) (+32%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 2.0 cm.
Plant dry weight*:
Control: 1.57 grams (c) Awaken: 2.34 grams (ab) (+23%) Vitazyme + Awaken: 2.56 grams (a) (+30%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 0.27 grams.
|
|
Fertilization: none |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2002 |
Blue Grass, Iowa |
Variety: Baldridge 705 silage corn Planting date: May 1, 2002 Seeding rate: 28,000 seeds/acre Soil type: unknown Row spacing: 30 inches Previous crop: soybeans Soil fertility: very high P and K |
Vitazyme application: 12 oz/acre of Vitazyme with the herbicide |
Corn Yield:
Control: 92.6 bu/acre The Vitazyme treated corn was about one foot taller than the control corn during much of the growing season.
|
|
Fertilization: Both areas received 100 lb/acre N plus 5 lb/acre sulfur at planting. Both areas also received 50 lb/acre of additional N sidedressed, with 5 lb/acre of sulfur. They received 5 gal/acre of Liquid Grow 8-19-3 starter in the furrow at planting. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2002 |
Cedar Falls, Iowa (Agricultural Research and Education Services; ACRES) |
Variety: Pioneer 33P67 Planting date: May 9, 2002 Seeding rate: 29,900 seeds/acre Soil type: Kenyon loam Row spacing: 30 inches Previous crop: soybeans Soil fertility: adequate P and K Planting depth: 1.5 inches |
Vitazyme application: 13 oz/acre in-furrow at planting (May 9) for the IF applications; 13 oz/acre over the soil (one week after planting May 16) for the BC application. Restore application: 2 oz/acre in-furrow at planting (May 9) for the IF application; 2 oz/acre over the soil (a week after planting on May 16) for the BC application. Bacteria application: 2 oz/acre in-furrow at planting (May 9) for the IF application; 2 oz/acre over the soil (a week after planting on May 16) for the BC application.
|
Mycorrhizae colonization (analyzed by Mycorrhizal Applications, Inc., Grants Pass, Oregon, using one root mass from each plot): Starter only (6-18-6): 33.0% (b) root colonization
Restore IF + Bacteria IF:
38.0% (ab) root colonization Vitazyme IF + Restore IF: 33.3% (a) root colonization Restore BC + Bacteria BC: 33.0% (b) root colonization *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 15.8%
|
|
Fertilization: The starter (6-18-6% N-P2O5-K2O) on all plots was applied with the seeds at planting, and the nitrogen was applied pre-plant as a 28% solution. |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2003 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: yellow dent Planting date: December 30, 2002 Planting rate: 10 seeds/pot, thinned to 3 plants/pot Soil type: Bowie very fine sandy loam Pot size: 1 gallon Replications: seven Harvest date: February 19, 2003 |
Vitazyme application: Vitazyme at 13 oz/acre |
Main Plots - Fertilizer effects Plant height*:
Control: 67.0 cm (c) 67% N: 112.2 cm (a) (+67%) 100% N: 115.8 cm (a) (+73%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 3.7 cm.
Dry weight*:
Control: 4.76 grams (d) 67% N: 16.09 grams (b) (+238%) 100% N: 18.92 grams cm (a) (+297%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 1.13 grams.
Sub Plots - Vitazyme effects Plant height*:
Control: 96.5 cm (a) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 2.5 cm.
Dry weight*:
Control: 11.93 grams (a) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 0.71 grams.
|
|
Fertilization: A 13-13-13% N-P2O5-K2O fertilizer plus other nutrients (0.65% Mg, 6.0% S, 0.0% B, 0.0006% Co, 0.06% Cu, 1.40% Fe, 0.06% Mn, 0.0006% Mo, and 0.06% Zn) were applied to Treatments 3 through 8 at rates of 33, 67, or 100% of the maximum rate of 40 lb/acre N. |
Replicated Study (Soil vs. Foliar Application)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2003 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: yellow dent Planting date: December 30, 2002 Planting rate: 10 seeds/pot, thinned to 3 plants/pot Soil type: Bowie very fine sandy loam Pot size: 1 gallon Replications: seven Harvest date: February 13, 2003 |
Vitazyme application: Vitazyme was applied to the soil surface only of Treatment 2 on January 24 about at the six-leaf stage. It was also applied (a spray of a 1% solution) to the leaf whorl of the plants of Treatment 3 on January 24; care was taken to avoid applying any product to the soil surface. |
Plant height*:
Control: 86.2 cm (a) Vitazyme on leaves: 94.5 cm (a) (+10%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 9.7 cm.
Plant dry weight*:
Control: 8.96 grams (b) Vitazyme on leaves: 10.51 grams (ab) (+17%) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 1.89 grams.
Both soil and foliar applications gave similar results for plant growth.
|
|
Fertilization: All plants were given 0.88 g/pot at planting of a pelleted 13-13-13% N-P2O5-K2O, slow release fertilizer with 0.65% Mg, 6.0% S, 0.0% B, 0.0006% Co, 0.06% Cu, 1.40% Fe, 0.06% Mn, 0.0006% Mo, and 0.06% Zn. This fertilizer gave an effective rate of 50 lb/acre of N, applied to the soil surface. |
Replicated Study (Surfactant Effects)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2003 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: yellow dent Planting date: December 30, 2002 Soil type: Bowie very fine sandy loam Pot size: 1 gallon Replications: five Harvest date: February 14, 2003 |
Vitazyme application: On January 24, 2003, Vitazyme at 1% was sprayed from a small spray bottle into the leaf whorl of all plants in Treatment 2, being careful not to apply to the soil surface. Paper towels were used to prevent any spray from contacting the soil of the pots. Vitazyme was also sprayed the same day on the leaves and whorl of Treatment 3, with a 1% Vitazyme solution plus 5 tablespoons/gallon of Sunspray Ultra-Fine Oil, a fine agricultural oil containing 98.8% paraffinic oil. |
Plant height*:
Control: 90.3 cm (b) Vitazyme foliar + surfactant: 91.2 cm (b) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 5.0 cm.
Plant dry weight*:
Control: 7.25 grams (b) Vitazyme foliar + surfactant: 9.88 grams (a) *Means followed by the same letter are not significantly different at P=0.10, according to the Tukey-Kramer Test. LSD0.10 = 1.58 grams.
A surfactant with Vitazyme did not improve crop response.
|
|
Fertilization: All plants received 0.88 g/pot at planting of a 13-13-13% N-P2O5-K2O pelleted fertilizer with 0.65% Mg, 6.0% S, 0.0% B, 0.0006% Co, 0.06% Cu, 1.40% Fe, 0.06% Mn, 0.0006% Mo, and 0.06% Zn. This fertilizer gave an effective rate of 50 lb/acre of N, applied to the soil surface. |
Testimonial
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2003 |
Sharon Springs, Kansas |
Variety: NC + 5021RB Planting date: May 3, 2003 Soil type: Keith sandy clay loam Planting rate: 26,000 seeds/acre Tillage system: no-till Previous crop: sunflowers Harvest date: October 10, 2003 Irrigation: center-pivot (16 inches of water during the growing season) |
Vitazyme application: (1) 13 oz/acre on May 7, with 0.5 lb/acre Atrazine 90df, 1.5 qt/acre Harness Extra, and 24 oz/acre Roundup herbicides; (2) 13 oz/acre on June 4, with 24 oz/acre Roundup herbicide when the corn was 10 inches tall |
Yield per acre for 120 acres: 229.2 bu/acre
Comments: The corn received significant hail damage on June 10 when the leaves were stripped. In spite of severe heat and wind as well, the corn did exceptionally well with Vitazyme, exceeding in yield any other fields in the area. Most fields in the area yielded 140 to 200 bu/acre, a few yielding 220 to 225 bu/acre.
|
|
Fertilization: 180 lb/acre N, 35 lb/acre P2O5 |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2003 |
Cedar Falls, Iowa (Agricultural Custom Research and Education Services; ACRES) |
Variety: Pioneer 34H1 non-GMO Planting date: May 22, 2003 Planting rate: 29,900 seeds/acre Soil type: Floyd loam Row spacing: 30 inches Planting depth: 1.5 inches Previous crop: soybeans Replications: four Harvest date: October 18, 2003 |
Vitazyme application: 13 oz/acre or 26 oz/acre, applied at planting on the seeds and at knee-height |
Grain yield*:
80 lb/acre N: 145.8 bu/acre
(b) 80 lb/acre N + Vitazyme twice, 26 oz/acre: 153.6 bu/acre (ab) (+5%) 160 lb/acre N: 159.9 bu/acre (a) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 8.0 bu/acre.
|
|
Fertilization: 80 to 160 lb/acre of N as a 28-0-0 solution; all other nutrients were adequate |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Clarkton, North Carolina (North Carolina State University) |
Variety: DKC69-71 RRH62 Planting date: April 24, 2004 Seeding rate: 33,000 seeds/acre Soil type: unknown Row spacing: 30 inches Previous crop: soybeans Harvest date: September 23, 2004 Tillage: conventional Reps: four |
Vitazyme application: Vitazyme at 13 oz/acre on the seeds at planting, plus Vitazyme at 13 oz/acre broadcast at knee height |
Corn Yield*:
Control, 0 N: 153.9 bu/acre
Control, 56 lb/acre N: 170.2 bu/acre
Control, 112 lb/acre N: 145.9 bu/acre
Control, 224 lb/acre N: 159.3 bu/acre
Control, 280 lb/acre N: 139.7 bu/acre Control, Average: 153.8 bu/acre Vitazyme, Average: 168.2 bu/acre* (+9% or 14.4 bu/acre) *Significantly different than the control at P=0.05.
Northern Corn Leaf Blight Rating*:
Control, 0 N: 9.25
Control, 56 lb/acre N: 9.38
Control, 112 lb/acre N: 9.63
Control, 224 lb/acre N: 9.75
Control, 280 lb/acre N: 10.00 bu/acre Control, Average: 9.60 Vitazyme, Average: 9.78 (+2%) *Significantly different than the control at P=0.05.
Gray Leaf Spot Rating*:
Control, 0 N: 8.88
Control, 56 lb/acre N: 8.88
Control, 112 lb/acre N: 9.00
Control, 224 lb/acre N: 9.38
Control, 280 lb/acre N: 9.50 bu/acre Control, Average: 9.13 Vitazyme, Average: 9.35* (+92%) *Significantly different than the control at P=0.05.
|
|
Fertilization: 10 gal/acre of 19-19-0 % N-P2O5-K2O in a 2x2 band on April 24 after broadcasting 30% UAN and a 10-34-0 fertilizer on April 19 |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Elizabeth City, North Carolina (North Carolina State University) |
Variety: DKC69-71 RRH62 Planting date: April 21, 2004 Seeding rate: 33,000 seeds/acre Soil type: unknown Row spacing: 30 inches Previous crop: soybeans Harvest date: unknown Tillage: conventional Reps: four |
Vitazyme application: Vitazyme at 13 oz/acre on the seeds at planting, plus 13 oz/acre broadcast at knee height |
Corn Yield*:
Control, 0 N: 54.0 bu/acre
Control, 56 lb/acre N: 118.3 bu/acre
Control, 112 lb/acre N: 157.8 bu/acre
Control, 224 lb/acre N: 150.0 bu/acre
Control, 280 lb/acre N: 139.9 bu/acre Control, Average: 124.0 bu/acre Vitazyme, Average: 142.7 bu/acre* (+15% or18.7 bu/acre) *Significantly different than the control at P=0.05.
Northern Corn Leaf Blight Rating*:
Control, 0 N: 3.25
Control, 56 lb/acre N: 3.75
Control, 112 lb/acre N: 3.88
Control, 224 lb/acre N: 4.25
Control, 280 lb/acre N: 4.13 Control, Average: 3.85 Vitazyme, Average: 5.35* (+39%) *Significantly different than the control at P=0.05.
Gray Leaf Spot Rating*:
Control, 0 N: 6.13
Control, 56 lb/acre N: 6.13
Control, 112 lb/acre N: 6.38
Control, 224 lb/acre N: 6.63
Control, 280 lb/acre N: 6.75 Control, Average: 6.40 Vitazyme, Average: 7.18* (+12%) *Significantly different than the control at P=0.05.
|
|
Fertilization: 10 gal/acre of 19-19-0 % N-P2O5-K2O in a 2x2 band on April 21 after broadcasting 30% UAN and a 10-34-0 fertilizer on April 19 |
Non-Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Austin, Texas |
Variety: Golden Acre 2850RR Planting date: March 28, 2004 Planting rate: 24,000 seeds/acre Soil type: silty clay loam Row spacing: 30 inches Previous crop: soybeans Harvest date: August 27, 2003 |
Vitazyme application: 13 oz/acre added to the seeds with liquid starter fertilizer at planting |
Grain yield:
Control: 131.9 bu/acre
|
|
Fertilization: anhydrous ammonia and a mixed N-P-K fertilizer |
Replicated Study (New Product Evaluation)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Cedar Falls, Iowa (Agricultural Custom Research and Education Services; ACRES) |
Variety: Pioneer 34M98 non-GMO Planting date: May 4, 2004 Seeding rate: 29,900 seeds/acre Soil type: Floyd loam Row spacing: 30 inches Previous crop: soybeans Replicates: six Planting depth: 1.5 inches Tillage: conventional Harvest date: October 21, 2004 |
Vitazyme application: 13 oz/acre applied at planting on the seeds, and again at knee height on June 15 (six leaves) |
Grain yield*:
Control: 163.0 bu/acre (b)
Product Y: 178.1 bu/acre (a)
(+9%) Product X + Product Y: 182.6 bu/acre (a) (+12%) *Means followed by the same letter are not significantly different according to the Student-Newman-Keuls Test.
Percent moisture:
Control: 17.51% (ab)
Product Y: 16.70 % (ab)
(-0.81
percentage point) Product X + Product Y: 18.47% (a) (+0.96 percentage point) *Means followed by the same letter are not significantly different according to the Student-Newman-Keuls Test.
Grain/plant*:
Control: 0.488 lb/plant (b)
Product Y: 0.515 lb/plant (b)
(+6%) Product X + Product Y: 0.553 lb/plant (a) (+13%) *Means followed by the same letter are not significantly different according to the Student-Newman-Keuls Test.
|
|
Fertilization: 100 lb/acre of N as a 28-0-0 solution; all other nutrients were adequate |
Non-Replicated Study (New Product Evaluation)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Gasport, New York |
Variety: Pioneer Liberty Link Planting date: June 15, 2004 Planting rate: 29,000 plants/acre (final) Soil type: Hilton loam Row spacing: 30 inches Harvest date: December 1, 2004 |
Vitazyme application: 13 oz/acre added to the seeds with the 9-18-9 liquid starter fertilizer at planting |
Grain yield:
Regular Vitazyme: 87.1 bu/acre
Decrease in grain moisture with Vitazyme Variant 1: -1.5 percentage points
|
|
Fertilization: 5 gal/acre of 9-18-9 (%N-P2O5-K2O) in the seed row at planting; 100 lb/acre (NH4)2SO4, 100 lb/acre pelleted lime, and 50 lb/acre of KCl preplant, and 25 gal/acre of 32% N side-dressed |
Replicated Study
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Hickory King Planting date: February 26, 2004 Seeding rate: 8 seeds/pot, thinned to 3 plants/pot Soil type: fine sandy clay loam Pot size: 1 gallon Replications: four Growing conditions: 60° to 85°F Harvest date: March 31, 2004 |
Vitazyme and Product 3 applications: Dilutions were prepared, and 100 ml of each product were added to the soil surface of each treatment after planting. Treatment 1 received only water. |
Plant height*:
Control: 83.5 cm (a) 0.0001% Product 3: 81.7 cm (ab) (-2%) 0.001% Product 3: 80.4 cm (ab) (-4%) 0.1% Product 3: 79.3 cm (ab) (-5%) 0.1% Vitazyme: 79.2 cm (ab) (-5%) 0.01% Product 3 + Vitazyme: 76.3 cm (b) (-9%) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 5.9 cm.
Plant dry weight*: 0.0001% Product 3: 10.23 grams (a) (+2%) 0.01% Product 3: 10.15 grams (ab) (+1%) 0.001% Product 3: 10.03 grams (ab) (+0%) Control: 10.02 grams (a)
0.1% Vitazyme: 9.29 grams (ab)
(-9%) 0.01% Product 3 + Vitazyme: 8.33 grams (b) (-17%) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 1.32 grams.
|
|
Fertilization: none |
Replicated Study (New Product Evaluation)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Cecilia, Kentucky |
Variety: NK 65M7 (non-GMO) Planting date: April 21, 2004 Planting rate: 20,000 plants/acre Soil type: Crider silt loam Row spacing: 30 inches Tillage: no-till Harvest date: October 4, 2004 |
New Vitazyme application: 13 oz/acre added to the seeds with the liquid starter fertilizer at planting |
Grain yield:
Control: 151.3 bu/acre Starter only: 158.4 bu/acre (+5%) New Vitazyme + Starter: 162.2 bu/acre (+7%)
|
|
Fertilization: 70 lb/acre of N, 90 lb/acre of P2O5 , and 90 lb/acre of K2O broadcast as a blended fertilizer before planting (in early April); a 5-20-5% N-P2O5-K2O starter fertilizer applied in the seed row at planting for Treatments 3 and 4; 1,000 lb/acre N as urea + Agritone (urease inhibitor), broadcast at 14-inch height in early June |
Replicated Study (New Product Evaluation)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Hickory King Planting date: February 4, 2004 Seeding rate: 8 seeds/pot, thinned to 3 plants/pot Soil type: fine sandy clay loam Pot size: 1 gallon Replications: six Growing conditions: 60° to 85°F Harvest date: March 18, 2004 |
Vitazyme and Product 2 applications: Dilutions were prepared, and 100 ml of each product (and of the combined products), were applied to the soil surface of each treatment after planting. Treatment 1 received only water. |
Plant height*:
0.001% Product 2: 76.4 cm (a)
(+6%) 0.01% Product 2: 75.2 cm (ab) (+4%) 0.1% Vitazyme: 75.2 cm (ab) (+4%) 0.0001% Product 2: 74.7 cm (ab) (+3%) 0.1% Product 2: 74.4 cm (ab) (+3%) 1% Product 2: 73.9 cm (ab) (+2%) Control: 72.3 cm (b) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 3.7 cm.
Plant dry weight*: 0.01% Product 2: 8.26 grams (a) (+1%) 1% Product 2: 8.17 grams (a) (+0%) Control: 8.16 grams (a) 0.1% Product 2: 8.14 grams (a) (-0%) 0.001% Product 2: 7.99 grams (a) (-2%)
0.1% Vitazyme: 7.92 grams (a)
(-3%) 0.0001% Product 2: 7.53 grams (a) (-8%) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 0.91 gram
|
|
Fertilization: none |
Replicated Study (New Product Evaluation)
|
Date |
Location |
Cultural System |
Vitazyme/Fertilization |
Results |
|
2004 |
Gladewater, Texas (Vital Earth Resources Research Center) |
Variety: Hickory King Planting date: February 3, 2004 Seeding rate: 8 seeds/pot, thinned to 3 plants/pot Soil type: fine sandy clay loam Pot size: 1 gallon Replications: four Growing conditions: 60° to 85°F Harvest date: March 17, 2004 |
Vitazyme and Product 1 applications: Dilutions were prepared, and 100 ml of each product were added to the soil surface of each treatment after planting. Treatment 1 received only water. |
Plant height*:
0.001% Product 1: 79.4 cm (a)
(+8%) 0.0001% Product 1 + Vitazyme: 76.7 cm (ab) (+4%) 0.1% Product 1: 76.6 cm (ab) (+4%) 0.01% Product 1 + Vitazyme: 76.0 cm (ab) (+4%) 0.01% Product 1: 74.8 cm (b) (+2%) 0.1% Product 1 + Vitazyme: 74.6 cm (bc) (+2%) 0.0001% Product 1: 74.1 cm (bc) (+1%) Control: 73.4 cm (bc) 0.1% Vitazyme: 70.4 cm (c) (-4%) 1% Product 1: 63.3 cm (d) (-15%) 1% Product 1 + Vitazyme: 56.3 cm (e) (-23%) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 4.5 cm.
Plant dry weight*:
0.001% Product 1: 10.00 grams
(a)
(+21%) 0.01% Product 1: 9.33 grams (ab) (+13%) 0.1% Product 1: 9.03 grams (ab) (+9%) 0.01% Product 1 + Vitazyme: 9.01 grams (b) (+9%) 0.0001% Product 1 + Vitazyme: 8.63 grams (bc) (+4%) Control: 8.27 grams (bc) 0.0001% Product 1: 8.25 grams (bc) (-0%) 0.1% Product 1 + Vitazyme: 7.74 grams (c) (-6%) 0.1% Vitazyme: 7.58 grams (c) (-8%) 1% Product 1: 6.28 grams (d) (-24%) 1% Product 1 + Vitazyme: 5.33 grams (d) (-36%) *Means followed by the same letter are not significantly different at P=0.10, according to the Student-Newman-Keuls Test. LSD0.10 = 0.98 gram
|
|
Fertilization: none |