Date

Location

Cultural System

Vitazyme/Fertilization

Results

1996

Tulia, Texas

Variety:  Paymaster HS-200

Planting date:  May 6, 1996

Seeding rate:  21 lb/acre

Harvest date:  November 10, 1996

Irrigation:  row

Vitazyme application:  Immediately after planting, 13 oz/acre of Vitazyme sprayed over the soil of Treatment 2.  A second application at 13 oz/acre was made to the foliage about two weeks after first bloom

Lint yield (Huseman Farm):

Control: 1088 lb/acre
Vitazyme: 1202 lb/acre (+10.5%)
 

Lint yield (McCormick Farm):

Control: 565 lb/acre
Vitazyme: 617 lb/acre (+9.1%)

Income increase (Huseman Farm):

+$114.00/acre
 

Income increase (McCormick Farm):

+$52.00/acre

Fertilization:  Composted manure at 2 tons/acre over all areas in October of 1995

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1996

Brownfield, Texas

Variety:  Paymaster HS-200

Planting date:  May 20, 1996

Seeding rate:  24 lb/acre

Row spacing:  40 inches

Harvest date:  October 14 to November 25

Irrigation:  center pivot

Vitazyme application:  Vitazyme was applied at 4 oz/acre directly on the seeds in the furrow at planting.  Another 4 oz/acre was applied by sprayer on July 20 and again on August 1 (12 oz/acre total)

Lint yield:

Control: 795 lb/acre
Vitazyme: 851 lb/acre (+7%)
 

Income increase:

+$56.00/acre

Fertilization:  10 tons/acre of manure before planting, 20 lb/acre of feather meal in the row before planting

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1997

Tulia, Texas

Variety:  Paymaster HS-200

Planting date:  May 14, 1997

Seeding rate:  22 lb/acre

Row spacing:  40 inches

Harvest date:  November 5, 1997

Irrigation:  row

Vitazyme application:  Vitazyme at 13 oz/acre on the seeds at planting, and at early bloom

Lint yield:

Control: 739 lb/acre

Vitazyme: 925.5 lb/acre (+25%)
 

Income increase:

+$103.01/acre

Fertilization:  2 tons/acre of steer compost in October, 1996

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1997

Miles, Texas

Planting date:  May 27, 1997

Seeding rate:  22 lb/acre

Row spacing:  40 inches (two planted, one skipped)

Previous crop:  sorghum

Soil type:  clayey

Harvest date:  October 10 and 17, 1997

Vitazyme application:  Vitazyme at 13 oz/acre on the seeds with the fertilizer at planting, and 13 oz/acre on the leaves and soil at early bloom

Yield increase:

Control: 350 lb/acre

Vitazyme: 375 lb/acre (+7%)

Income increase:

+$15.50/acre

Fertilization:  At planting, 120 lb/acre of a 15-15-0 liquid fertilizer applied on the seeds

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1997

Littlefield, Texas

Planting date:  May 20, 1997

Variety:  Alltex Atlas

Seeding rate:  18 lb/acre

Row spacing:  40 inches

Previous crop:  cotton

Soil type:  deep fine sandy loam

Irrigation:  center pivot

Temik treatment:  Temik was applied at the recommended rate at planting to the half of the plot that did not receive Vitazyme.

Vitazyme application:  At planting, 13 oz/acre was applied along with Direx herbicide in a 10-inch band over each seed row.  At pinhead square, 13 oz/acre was applied to the leaves and soil.

Lint yield:

Temik: 642.2 lb/acre (+4.6%)
Vitazyme: 612.2 lb/acre

[Note: Although the Temik treatment yielded slightly more, this test showed that Vitazyme can overcome pathogenic nematode problems as well as highly toxic Temik.  Vitazyme produced darker green, leafier plants than did Temik.]

Fertilization:  Some liquid starter was applied to the soil surface before planting, and 65 lb/acre of anhydrous ammonia was banded at the end of June.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1998

Whitharral, Texas

Variety:  Paymaster HS-26 (Roundup Ready)

Seeding rate:   16 lb/acre

Planting date:  May 21, 1998

Soil type:  fine sandy loam

Row spacing:  40 inches

Harvest date:  October 21, 1998

Irrigation:  center pivot

Temik treatment:  Temik was applied at the recommended rate at planting to the half of the plot that did not receive Vitazyme.

Vitazyme application:  Vitazyme was sprayed at 13 oz/acre over the soil surface directly behind the planter.  A second application of 13 oz/acre was sprayed on the leaves and soil the second week of June.

Lint Yield:

Temik: 669 bu/acre
Vitazyme: 711 bu/acre (+6%)

[Note: Vitazyme did somewhat better at enhancing lint production than did Temik.]

Income Increase:

+$22.68/acre

Fertilization:  Two applications of 40 lb/acre nitrogen were made through the center pivot in June.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Parma, Missouri

Variety:  Stoneville 373

Seeding rate:   14 lb/acre

Planting date:  May 13, 1999

Previous crop:  corn

Row spacing:  38 inches

Vitazyme application:  (1) Vitazyme at 13 oz/acre sprayed over the row in a 19-inch band with preemergent chemicals;  (2) 13 oz/cre over the leaves at early bloom.

Cotton fiber evaluation (yield differences were not significant, though Vitazyme yielded more than the control):

Lint Yield:

Control: 32.7%
Vitazyme: 33.4%

Fiber Strength:

Control: 24.4 g/tex
Vitazyme: 26.4 g/tex

Micronaire:

Control: 4.9
Vitazyme: 4.4

[Note: Vitazyme produced superior fibers.]

Fertilization:  nitrogen, 100 lb/acre; phosphate, 40 lb/acre; potash, 60 lb/acre

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Littlefield, Texas

Variety:  "Excess"

Planting date:  May 21, 1999

Seeding rate:   18 lb/acre

Previous crop:  cotton

Row spacing:  40 inches

Irrigation:  row-irrigated

Soil type:  sandy loam

Harvest date:  October 26, 1999

Vitazyme application:  Vitazyme at 13 oz/acre in a 10-inch band over the seed row at planting;  Vitazyme at 13 oz/acre sprayed on the leaves with Vydate (for boll weevils) on July 10

Lint Yield:

Control: 402 lb/acre
Vitazyme: 425 lb/acre (+6%)

Income Increase:

+$13.80/acre

Fertilization:  60 lb N/acre knifed in between the rows on June 25

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Blackville, South Carolina (Clemson University)

Variety:  Delta Pine 5415 Roundup Ready

Planting date:  May 14, 1999

Seeding rate:   3 seeds/foot

Previous crop:  cotton

Row spacing:  38 inches

Irrigation:  4 times by traveling gun (June 7, 0.5 in; July 26, 0.5 in;  August 2, 0.25 in;  August 12, 0.25 in)

Soil type:  Varina loamy sand

Insecticide:  5 lb/acre of Temik at planting

Harvest date:  October 15, 1999

Replications:  four

Vitazyme application:  13 oz/acre on the seeds at planting (May 14);  13 oz/acre on the leaves at first bloom (July 19)

Leaf N:

Control: 3.91%
Vitazyme: 4.22%* (+8%)

*Significantly greater than the control at P=0.05.

Leaf P:

Control: 0.29%
Vitazyme: 0.31%* (+7%)

*Significantly greater than the control at P=0.05.

Leaf K:

Control: 1.88%
Vitazyme: 2.08%* (+16%)

*Significantly greater than the control at P=0.05.

[Note: Calcium, magnesium, and sulfur were also significantly increased by Vitazyme, each by 4%.]

Lint yield:

Control: 1,052 lb/acre
Vitazyme: 1,083 lb/acre* (+3%)

*Significantly greater than the control at P=0.05.

Soil NO₃-N at 12 weeks:

Control: 10.07 ppm
Vitazyme: 11.32 ppm* (+12%)

*Significantly greater than the control at P=0.05.

Plant height at 12 weeks:

Control: 32.6 inches
Vitazyme: 33.1 inches* (+2%)

*Significantly greater than the control at P=0.05.

Fertilization:  100 lb K₂O/acre before planting;  90 lb N/acre in increments of 30 lb N/acre, three times during the season

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Tulia, Texas

Variety:  Paymaster HS-200

Planting date:  May 13, 1999

Seeding rate:   22 lb/acre

Row spacing:  40 inches

Irrigation:  all plots were furrow irrigated

Soil type:  sandy loam

Harvest date:  November 6, 1999

Vitazyme application:  Vitazyme was sprayed on the soil at 13 oz/acre on May 5, two days after planting, and at early bloom.

Lint Yield:

Control: 497 lb/acre
Vitazyme: 655 lb/acre (+32%)

Income Increase:

+$110.30/acre

Fertilization:  2 tons/acre of steer compost in October, 1998

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Littlefield, Texas

Variety:  Paymaster HS-26

Planting date:  May 16, 1999

Seeding rate:   16 lb/acre

Row spacing:  40 inches

Previous crop:  cotton

Irrigation:  twice, 1.5 inches each time

Soil type:  sandy loam

Harvest date:  November 15, 1999

Irrigation:  center pivot

Vitazyme application:  Vitazyme was mixed with Direx at 13 oz/acre and applied over the seed row in a 10-inch band at planting.  A second application was made June 22 at 13 oz/acre, band sprayed (10 inches wide) over the rows.

Lint yield:

Control: 512 lb/acre
Vitazyme: 559 lb/acre (+9%)

Income increase:

+$28.20/acre

Fertilization:  Preplant: 11-52-11-11-5 lb/acre of N-P₂O₅-K₂O-S-Mn;  a side-dress application of 150 lb/acre of 32-0-0

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Littlefield, Texas

Variety:  Paymaster HS-26

Planting date:  May 5, 1998

Seeding rate:   20 lb/acre

Row spacing:  40 inches

Previous crop:  cotton

Soil type:  medium sandy loam

Harvest date:  November 7, 1999

Irrigation:  center pivot

Temik application:  4 lb/acre on the seeds

Vitazyme application:  (1) 13 oz/acre in a 10-inch band over the row at planting;  (2) 13 oz/acre sprayed over the leaves and soil at early bloom

Note:  This was on top of a Temik application

Lint yield:

Temik 761.6 lb/acre
Vitazyme+Temik: 793.6 lb/acre (+4%)

[Note: Vitazyme improved lint yield when used in addition to Temik.]

There was little difference in fiber quality for the two treatments.

Income Increase:

+$19.20/acre

Fertilization:  30-40-0 lb/acre N-P₂O₅-K₂O preplant liquid, with Zn and B.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Mississippi State, Mississippi

(Mississippi State University)

Baton Rouge, Louisiana

(Louisiana State University)

Plano, Texas

(Gustafson)

College Station, Texas

(Texas A&M University)

Variety:  unknown

Vitazyme application:  (1) Lot 1.  Regular Vitazyme (10%), with seeds soaked for five minutes and then air dried.

(2) Lot 2.  Autoclaved Vitazyme (10%), with seeds soaked for five minutes and then air dried.  Autoclaving was done at 15 lb/in² pressure for 15 min at 121 C, and reautoclaved one day later.

Texas (Texas A&M University)

Number of plants:

Control: 24.8
Vitazyme regular: 56.0*

Vitazyme autoclaved: 52.6*

*Significantly greater than the control at P=0.01.

Lousiana (Louisiana State University)

Number of plants:

Control: 4.6
Vitazyme regular: 10.8*

Vitazyme autoclaved: 13.4**

*Significantly greater than the control at P=0.06.

**Significantly greater than the control at P=0.04.

Texas (Gustafson)

Number of plants:

Control: 57.6
Vitazyme regular: 61.3

Vitazyme autoclaved: 60.3

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Mississippi State, Mississippi

(Mississippi State University)

Fayetteville, Arkansas

(University of Arkansas)

Plano, Texas

(Gustafson)

College Station, Texas

(Texas A&M University)

Auburn, Alabama

(Auburn University)

Knoxville, Tennessee

(University of Tennessee)

Variety:  unknown

Replications:  four

Vitazyme application:  (1) Lot 1.  Regular Vitazyme (10%), with seeds soaked for five minutes and then air dried.

(2) Lot 2.  Autoclaved Vitazyme (10%), with seeds soaked for five minutes and then air dried.  Autoclaving was done at 15 lb/in² pressure for 15 min at 121 C, and reautoclaved one day later.

Alabama (Auburn University)

Number of plants:

Control: 68.8
Vitazyme: 72.6 (+6%)

Arkansas (University of Arkansas)

Number of plants:

Control: 44.2
Vitazyme: 45.6 (+3%)

Mississippi (Mississippi State University)

Number of plants:

Control: 70.5
Vitazyme: 75.9 (+8%)

Tennessee (University of Tennessee)

Number of plants:

Control: 31.2
Vitazyme: 35.0 (+12%)

Texas (Texas A&M University)

Number of plants:

Control: 51.7
Vitazyme: 55.4 (+7%)

Texas (Gustafson)

Number of plants:

Control: 57.4
Vitazyme: 62.2 (+8%)

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Tulia, Texas

Variety:  HS 2200 (Paymaster Roundup Ready)

Planting date:  May 6, 2000

Row spacing:  38 inches

Soil type:  sandy loam

Harvest date:  October 5, 2000

Irrigation:  one time by center pivot shortly after plant emergence

Vitazyme application:  13 oz/acre banded on top of the soil of the seed row at planting, and 13 oz/acre at early bloom

Lint yield:

Control: 220.1 lb/acre
Vitazyme: 241.3 lb/acre (+10%)

Lint quality improvement with Vitazyme:

Fiber strength: +4%
Fiber uniformity: +1%

Fiber length: +4%

Income increase:

+$13.55/acre

Fertilization:  Last October (1999), 2 tons/acre of steer compost was applied over the entire area.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Littlefield, Texas

Variety:  HS-26, Roundup Ready

Planting date:  May 29, 2000

Soil type:  medium sandy loam

Harvest date:  November 19 to 23, 2000

Irrigation:  center pivot

Vitazyme application:  13 oz/acre in a 12-inch band over the rows at planting, and 13 oz/acre sprayed over the leaves and soil near early bloom with the first boll weevil spray

Lint yield:

Control: 460.4 lb/acre
Vitazyme: 552.5 lb/acre (+20%)

Income increase:

+$55.26/acre

Fertilization:  55 lb/acre N, 40 lb/acre P₂O₅, and trace elements were applied before planting and incorporated into the soil

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Lamesa, Denver City, and Gomez, Texas

Variety:  unknown

Lamesa planting date:  May 8, 2000

Denver City planting date:  May 23, 2000

Gomez Planting date:  May 5, 2000

Planting rate:  unknown

Variety:  unknown

Row spacing:  38 inches

Harvest date:  varaible

Irrigation:  center pivot

Vitazyme application:  13 oz/acre in the sed row at planting; 13 oz/acre sprayed at pinhead square

Temik application:  Temik 15 G at 5 lb/acre in the furrow

Lamesa plots - Nematode counts

Eggs per cc of soil:

Control: 12,870

Temik: 11,177
Vitazyme: 5,730

Juveniles per cc of soil:

Control: 65

Temik: 20
Vitazyme: 55

Denver City plots - Nematode counts

Eggs per cc of soil:

Control: 3,165

Temik: 1,170
Vitazyme: 2,925

Juveniles per cc of soil:

Control: 5

Temik: 5
Vitazyme: 30

Gomez plots - Nematode counts

Eggs per cc of soil:

Control: 1,965

Temik: 1,170
Vitazyme: 1,020

Juveniles per cc of soil:

Control: 85

Temik: 35
Vitazyme: 30

Plot Summary: Vitazyme gave results similar to Temik for nematode effects.

Eggs per cc of soil:

Temik: -38%
Vitazyme: -37%

Juveniles per cc of soil:

Temik: -43%
Vitazyme: -40%

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Mississippi State, Mississippi

(Mississippi State University)

West Lafayette, Indiana

(Purdue University)

Plano, Texas

(Gustafson)

College Station, Texas

(Texas A&M University)

Auburn, Alabama

(Auburn University)

Variety:  unknown

Replications:  four

Vitazyme application:  (1) Lot 1.  Regular Vitazyme (10%), with seeds soaked for five minutes and then air dried.

(2) Lot 2.  T-22 (1 oz/gallon of soluble powder), with seeds soaked for five minutes and then dried.

(3) Lot 3.  Vitazyme (10%) + T-22 (1 oz/gallon), with seeds soaked for five minutes and then dried.

(4) Lot 4.  Autoclaved Vitazyme (10%), with seeds soaked for five minutes and then air dried.  Autoclaving was done at 15 lb/in² pressure for 15 min at 121 C, and reautoclaved one day later.

Alabama (Auburn University)

Number of plants:

Control: 12.0

Vitazyme: 13.4 (+12%)

T-22: 13.0 (+8%)

Vitazyme + T-22: 9.4 (-22%)

Vitazyme autoclaved: 12.2 (+2%)

Indiana (Purdue University)

Number of plants:

Control: 3.6

Vitazyme: 3.0 (-17%)

T-22: 4.2 (+17%)

Vitazyme + T-22: 3.8 (+6%)

Vitazyme autoclaved: 2.8 (-22%)

Mississippi (Mississippi State University)

Number of plants:

Control: 72.8

Vitazyme: 68.6 (-6%)

T-22: 73.4 (+1%)

Vitazyme + T-22: 77.1 (+6%)

Vitazyme autoclaved: 77.6 (+7%)

Texas (Texas A&M University)

Number of plants:

Control: 44.6

Vitazyme: 44.6 (+0%)

T-22: 52.2 (+17%)

Vitazyme + T-22: 47.0 (+5%)

Vitazyme autoclaved: 48.8 (+9%)

Texas (Gustafson)

Number of plants:

Control: 10.6

Vitazyme: 9.4 (-9%)

T-22: 13.6 (+28%)

Vitazyme + T-22: 15.8 (+49%)

Vitazyme autoclaved: 9.4 (-9%)

Fertilization:  unknown

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Lubbock, Texas

(Texas A&M University Research and Extension Center)

Variety:  Paymaster 2326RR + T-22

Planting date:  May 16, 2001

Row spacing:  36 inches

Soil type:  unknown

Planting rate:  standard for the area

Irrigation:  center pivot

Vitazyme application:  (1) 13 oz/acre with the seeds at planting; (2) 13 oz/acre sprayed in 10 gal/acre of water over the leaves and soil at the pinhead square stage on June 22.

Gaines County Site

Plant height:

Control: 16.0 in
Temik: 16.3 in (+2%)

Vitazyme: 16.5 in (+3%)

Primary boll number:

Control: 4.0
Temik: 3.7 (-8%)

Vitazyme: 4.3 (+8%)

Secondary boll number:

Control: 1.1
Temik: 0.9 (-18%)

Vitazyme: 1.0 (-9%%)

Node number:

Control: 12.1
Temik: 12.6 (+4%)

Vitazyme: 12.4 (+2%)

Denver County Site

Nematode number versus the control:

Temik: -22%
Vitazyme: -46%

Vitazyme gave results similar to, or superior to, Temik for nematode effects at the two sites.

Fertilization:  standard for the area

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Whitharral, Texas

Variety:  Stoneville 2454, Roundup Ready

Planting date:  May 15, 2002

Row spacing:  40 inches

Soil type:  sandy loam

Harvest date:  October 3, 2002

Irrigation:  center pivot

Vitazyme application:  (1) 13 oz/acre in a 10-inch band behind each row at planting;  (2) 13 oz/acre sprayed on the soil and leaves at the pinhead square stage (about July 8)

Lint yield:

Control: 886 lb/acre
Vitazyme: 1,131 lb/acre (+28%)

Cotton income:

Control: $509.45/acre
Vitazyme: $650.33/acre (+$140.88/acre)

Cost:Return: 17.6:1

Fertilization:  A preplant application of N and P were made, and 500 gal of a 11-52-0% N-P₂O₅-K₂O formulation was distributed through the center pivot system for all areas.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Gladewater, Texas

(Vital Earth Resources Research Greenhouse)

Variety:  cotton (not specified)

Planting date:  March 19, 2002

Seeding rate:  10 seeds/pot, thinned to 3 seeds/pot

Soil type:  Bowie very fine sandy loam

Pot size:  1 gallon

Harvest date:  May 7, 2002, 49 days after planting.

Vitazyme application:  After planting on February 22, 50 ml of a 0.002% Vitazyme solution was applied to the soil surface of each pot for Treatment 2.  This application was equal to the amount of Vitazyme contained in the Granusol-Mn* of Treatment 4.

*Granusol-Mn is a sucrate carboxylate containing a simple carbohydrate binder, together with various minerals (Mn, 35%; CaO, 11%; Fe, 4%; SO₃, 0.8%; Zn, 0.3%), with granules able to quickly break down in water to supply nutrients to plants.  Producer: American Minerals, Dunedin, Florida.

Plant height:

Control: 21.8 cm

Vitazyme: 23.9 cm* (+9%)

Granusol-Mn: 23.1 cm*
Vitazyme+Granusol-Mn: 24.7 cm* (+13%)

*Significantly greater than the control at P=0.10, according to the Student-Newman-Keuls Test.  LSD_0.10=1.4 cm.
 

Plant dry weight:

Control: 2.31 grams

Vitazyme: 3.13 grams* (+35%)

Granusol-Mn: 2.97 grams*
Vitazyme+Granusol-Mn: 3.36 grams* (+45%)

*Significantly greater than the control at P=0.10, according to the Student-Newman-Keuls Test.  LSD_0.10=0.45 grams.

Fertilization:  Each pot received 0.23 gram per pot of (NH₄)₂SO₄ to equal a 100 lb/acre application, or 21 lb/acre of N and 20 lb/acre of S for a "starter" effect.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Lorenzo, Texas

(Texas A&M Research and Education Center)

Variety:  Paymaster 2326 BT/RR

First planting date:  May 27, 2003

Planting rate:  unknown

Row spacing:  40 inches

Harvest date:  December 10, 2003

Vitazyme application:  13 oz/acre, in-furrow at planting, and again foliar/soil at 13 oz/acre after planting on July 22, 2003

Lint yield:

Control: [base yield for calculation]
Vitazyme: + 33.5 lb/acre (+4%)

[Temik results were similar.]

Income increase:

+$12.10/acre)

Fertilization:  the same across all areas