Date

Location

Cultural System

Vitazyme/Fertilization

Results

1997

New Iberia, Louisiana

Variety:  LCP 85-384

Planting date:  mid-December, 1996

Harvest date:  December 9 and 10, 1997

Vitazyme application:  (1) Vitazyme at 13 oz/acre on the seed pieces at planting;

(2) Vitazyme at 13 oz/acre on the soil and leaves at emergence;

(3) Vitazyme at 13 oz/acre on the leaves and soil at layby (18 to 24-inches height).

Sugar Yield:

Control: 7,406 lb/acre
Vitazyme: 7,430 lb/acre (little change)
 

Millable Stalks:

Control: 46,200 stalks/acre
Vitazyme: 50,900 stalks/acre (+10%)

Fertilization:  Starter mix at planting:  18-36-50-18 lb/acre N-P₂O₅-K₂O-S.  In April, 120-36-62-20 lb/acre N-P₂O₅-K₂O-S.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Santiago de Cuba, Cuba

Variety:  C89-147

Type:  ratoon

Soil type:  Leptic haplustert

Vitazyme application:  1 liter/ha three times, monthly from the last harvest, for Treatments 3 and 4

[Percentage increases compare responses to the no nitrogen control.]

Stalk length:

Control: 210 cm
100% N: 214 cm (+2%)

Vitazyme + 50% N: 214 cm (+2%)

Vitazyme + 100% N: 216 cm (+3%)
 

Stalk diameter:

Control: 2.68 cm
100% N: 2.85 cm (+6%)

Vitazyme + 50% N: 2.81 cm (+5%)

Vitazyme + 100% N: 2.87 cm (+7%)

Cane Yield*:

Control: 82.99 tons/ha (b)
100% N: 87.70 tons/ha (b) (+6%)

Vitazyme + 50% N: 106.66 tons/ha (ab) (+29%)

Vitazyme + 100% N: 112.89 tons/ha (a) (+36%)

*Means followed by the same letter are not significantly different.  Standard error (sugar yield) = 0.53 t/ha; standard error (cane yield) = 4.59 t/ha.

Sugar Yield*:

Control: 11.95 tons/ha (c)
100% N: 12.21 tons/ha (c) (+2%)

Vitazyme + 50% N: 15.05 tons/ha (b) (+26%)

Vitazyme + 100% N: 16.50 tons/ha (a) (+38%)

*Means followed by the same letter are not significantly different.  Standard error (sugar yield) = 0.53 t/ha; standard error (cane yield) = 4.59 t/ha.

Fertilization:  Nitrogen was applied at 75 kg/ha for Treatments 2 and 4, and at 37.5 kg/ha for Treatment 3.  The control (Treatment 1) received no fertilizer, as recommended for the crop cycle and soil of this study.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Espana Rep. Estate, Mantanzas Province, Cuba

Variety:  Mantanzas C323-68

Type:  ratoon

Soil type:  Eutrustox (Ferralsol)

Vitazyme application:  1 liter/ha three times broadcast monthly from the last harvest for Treatment 2, and five times for Treatment 3

Cane Yield*:

100% Fertilizer: 54.27 tons/ha
Vitazyme + 100% Fertilizer: 62.09 tons/ha (+14%)

Vitazyme + 75% Fertilizer: 61.38 tons/ha (+13%)

Sugar Yield*:

100% Fertilizer: 9.87 tons/ha (a)
Vitazyme + 100% Fertilizer: 10.89 tons/ha (b) (+10%)

Vitazyme + 75% Fertilizer: 10.81 tons/ha (b) (+10%)

*Means followed by the same letter are not significantly different.  Standard error = 1.25 tons/ha

Fertilization:  Fertilizer was applied at 100 or 75% of recommended rates to all treatments (100%: 130 kg/ha N, 100 kg/ha K₂O; 75%: 97.5 kg/ha N, 75 kg/ha K₂O).

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Holguin, Cuba

Variety:  C323-68

Type:  ratoon

Soil type:  Calciustert

Vitazyme application:  Vitazyme was applied at various rates ranging from 0.5 to 1.0 liter/ha, and 0, 3, or 5 times.  Total cumulative applications were 0, 1.5, 2.5, 3, or 5 liters/ha.  Placement was either banding or broadcasting.

[Percent increases are from a comparison with the no nitrogen control treatment.]

Cane Yield:

Control:  53.43 tons/ha

Vitazyme (1 l/ha x 5) + 100% fertilizer: 78.43 tons/ha (a) (+47%)
Vitazyme (1 l/ha x 3) + 100% Fertilizer: 75.16 tons/ha (ab) (+41%)

Vitazyme (0.5 l/ha x 5) + 100% fertilizer: 76.47 tons/ha (ab) (+43%)
Vitazyme (1 l/ha x 3) + 100% Fertilizer: 65.67 tons/ha (ab) (+23%)

Sugar Yield*:

Vitazyme (1 l/ha x 5) + 100% fertilizer: 12.06 tons/ha (+62%)
Vitazyme (1 l/ha x 3) + 100% Fertilizer: 11.30 tons/ha (+52%)

Vitazyme (0.5 l/ha x 5) + 100% fertilizer: 10.95 tons/ha (+47%)
Vitazyme (1 l/ha x 3) + 100% Fertilizer: 9.67 tons/ha (+30%)

Added sugar value (at $0.07/lb of sugar):

100% fertilizer, no Vitazyme: +$279.28

Vitazyme (1 l/ha x 5 applications) + 100% fertilizer: +$561.65
Vitazyme (1 l/ha x 3 applications) + 100% Fertilizer: +$473.70

Vitazyme (0.5 l/ha x 5 applications) + 100% fertilizer: +$430.50
 

Extra cost per dollar of added sugar (at $0.07/lb):

100% fertilizer, no Vitazyme: $0.73

Vitazyme (1 l/ha x 5 applications) + 100% fertilizer: $0.55
Vitazyme (1 l/ha x 3 applications) + 100% Fertilizer: $0.57

Vitazyme (0.5 l/ha x 5 applications) + 100% fertilizer: $0.65

Overall Vitazyme effects exceeded the controls for stalk diameter, stalk length, stalk population, stalk weight, juice purity, cane yield, sugar content, and sugar yield.

Fertilization:  100% recommendation: 130 kg/ha N and 176 kg/ha P₂O₅/acre.  75% and 50% rates were also applied.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Santiago de Cuba, Cuba

Variety:  C86-12

Type:  new planting

Soil type:  Haplustert

Row spacing:  1.6 meters

Vitazyme application:  1 liter/ha (13 oz/acre) monthly from planting.  Vitazyme was applied at various rates ranging from 0.5 to 1.0 liter/ha, and 0, 2, 3, or 5 times.  Total cumulative applications were 0, 1.5, 2.5, 3, or 5 liters/ha.  Placement was either banding or broadcasting, or on the seed pieces at 1, 2.5, or 5 % as a soak.

Cane Yield**:

Control:  49.64 (b)

Vitazyme (1 l/ha x 3), broadcast: 58.31 tons/ha (a) (+18%)
Vitazyme (1 l/ha x 5), broadcast: 57.81 tons/ha (a) (+17%)

Vitazyme (0.5 l/ha x 5), band: 55.79 tons/ha (ab) (+12%)

Vitazyme (0.5 l/ha x 3), band: 57.22 tons/ha (a) (+15%)
Vitazyme (2.5% +1* x 2), broadcast: 56.44 tons/ha (ab) (+14%)

*Cane sets were soaked in a 2.5% Vitazyme solution (v/v) for 5 minutes before planting.

**Means followed by the same letter are not significantly different at P=0.05 according to Duncan's Multiple Range Test.

Sugar Yield*:

Control:  6.23 tons/ha

Vitazyme (1 l/ha x 3), broadcast: 7.40 tons/ha (+19%)
Vitazyme (1 l/ha x 5), broadcast: 7.30 tons/ha (+17%)

Vitazyme (0.5 l/ha x 5), band: 7.20 tons/ha (+16%)

Vitazyme (0.5 l/ha x 3), band: 7.03 tons/ha (+13%)
Vitazyme (2.5% +1* x 2), broadcast: 7.00 tons/ha (+12%)

*Cane sets were soaked in a 2.5% Vitazyme solution (v/v) for 5 minutes before planting.

Added value of sugar (at $0.07/lb of sugar):

Vitazyme (1 l/ha x 3), broadcast: +$180.53
Vitazyme (1 l/ha x 5), broadcast: +$165.10

Vitazyme (0.5 l/ha x 5), band: +$149.67

Vitazyme (0.5 l/ha x 3), band: +$123.44
Vitazyme (2.5% +1* x 2), broadcast: +$118.81

*Cane sets were soaked in a 2.5% Vitazyme solution (v/v) for 5 minutes before planting.

Extra cost per dollar of added sugar (at $0.07/lb):

Vitazyme (1 l/ha x 3), broadcast: $0.37
Vitazyme (1 l/ha x 5), broadcast: $0.54

Vitazyme (0.5 l/ha x 5), band: $0.41

Vitazyme (0.5 l/ha x 3), band: $0.41
Vitazyme (2.5% +1* x 2), broadcast: $0.64

*Cane sets were soaked in a 2.5% Vitazyme solution (v/v) for 5 minutes before planting.

Overall Vitazyme effects exceeded the controls for cane yield, sugar content, and sugar yield.

Fertilization:  None was needed in the plant cane cycle investigated, and in the soil type utilized, as recommended in Cuba for newly planted cane.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Manuel Fajardo Estate, Havana Province, Cuba

Variety:  Co 997

Type:  ratoon

Soil type:  Eutrustox

Vitazyme application:  1liter/ha three times for Treatment 3 and five times for Treatment 4, monthly after the last harvest

Juice purity:

Control: 93.50%
100% fertilizer: 91.81% (-2%)

Vitazyme (1 l/ha x 3) + 50% fertilizer: 95.04% (+2%)
Vitazyme (1 l/ha x 5) + 100% fertilizer: 93.25% (0%)

Cane yield:

Control: 41.5 tons/ha
100% fertilizer: 46.3 tons/ha (+12%)

Vitazyme (1 l/ha x 3) + 50% fertilizer: 42.2 tons/ha (+2%)
Vitazyme (1 l/ha x 5) + 100% fertilizer: 48.2 tons/ha (+16%)

Sugar yield:

Control: 6.23 tons/ha
100% fertilizer: 6.63 tons/ha (+6%)

Vitazyme (1 l/ha x 3) + 50% fertilizer: 6.44 tons/ha (+3%)
Vitazyme (1 l/ha x 5) + 100% fertilizer: 7.43 tons/ha (+19%)

Sugar content:

Control: 15.01%
100% fertilizer: 14.33% (-5%)

Vitazyme (1 l/ha x 3) + 50% fertilizer: 15.27% (+2%)
Vitazyme (1 l/ha x 5) + 100% fertilizer: 15.42% (+3%)

Fertilization:  130 kg/ha N and 100 kg/ha K₂O for the 100% rate, and half of those values for the 50% rate