Vitazyme
Field Trial Results: 1995-Present
Tomatoes
 
Testimonial

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1997

Firebaugh, California

Variety:  Heinz 8892 paste tomatoes

Planting date:  April 16, 1997

Soil type:  unknown

In-row spacing:  unknown

Harvest date:  September 15, 1997

Vitazyme application:  Vitazyme at 12 oz/acre was added directly to the seeds at planting with a starter, and again at 5 oz/acre, sprayed over the top of the plants at the end of April.

Comments:

According to the farmer, "We were unfortunately unable to separate the yield of the Vitazyme section of the field from an adjoining 30-row section that had Kwik-Start (7-21-0), or from any untreated areas of the field.  These early tomatoes were used to mix with other tomatoes that had more rot, to improve the overall grade.  I would estimate the yield improvement of Vitazyme and Kwik-Start compared to the regular planting as follows:
Vitazyme:  2 to 4 tons/acre ($100 to $200/acre)

Kwik-Start:  1 to 2 tons/acre ($50 to $100/acre)

Besides the improved yield, the Vitazyme treated tomatoes were firmer, had fewer green fruit, and were more uniform in size than the other parts of the field."

 

Leaf Chlorophyll (north end of field):

Control: 53.2 SPAD units
Vitazyme: 55.5 SPAD units
(+2.3 SPAD units)

 

Leaf Chlorophyll (south end of field):

Control: 55.6 SPAD units
Vitazyme: 56.5 SPAD units
(+0.9 SPAD unit)

Fertilization:  A 40 gallon/acre 10-34-0 preplant fertilizer application was made;

40 gallons/acre of UN-32 was sprayed over the top of the plants at the end of April.

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1998

Crows Landing, California

Variety:  Roma (canning type)

Planting date:  unknown

Bed spacing:  60 inches (double row)

Vitazyme application:  (1) Vitazyme at 13 oz/acre mixed with sidedress fertilizer at about 6 to 8 inches in height (about early bloom);  (2) Vitazyme at 13 oz/acre sprayed with a fungicide at fruit filling.

Sample Weight:

Control: 28.5 lb
Vitazyme: 34.0 lb
(+19%)

 

Leaf Chlorophyll:

Control: 50.6 SPAD units
Vitazyme: 55.6 SPAD units
(+5.0 SPAD units)

 

Yield Increase:

+2,100 lb/acre

 

Income Increase:

+$58.30/acre

Fertilization:  conventional for the entire area

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Trinidad, West Indies (Caribbean Agricultural Research and Development Institute)

Variety:  Kada hybrid

Planting date:  November 10, 1998

Planting rate:  14 plants/6 meter row

In-row spacing:  2.3 plants/meter

Harvest date:  February 2, 1999

Vitazyme application:  (1) Root dip at planting, using 0.5% Vitazyme; (2) Vitazyme at 1 liter/ha sprayed on the leaves and soil at early bloom

Tomato Weight:

Control: 635.0 kg/plot
Vitazyme: 661.0 kg/plot
(+4%)

 

Yield Increase:

+6,433 lb/acre

 

Income Increase:

+$1,929.90/acre

Fertilization:  Planting to early bloom:  28 g/plant each week of a 12-12-17-2% N-P2O5-K2O fertilizer.

Flowering to the end of the trial:  28 g/plant each week of a 13-13-21 N-P2O5-K2O fertilizer

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Trinidad, West Indies

Variety:  Kada

Planting date:  April 6, 1999

Harvest date:  unknown

Vitazyme application:  The 15, 20, and 30 ml/gal rates were applied at three times:  (1) at transplanting to the soil and foliage;  (2) two weeks after transplanting to the foliage;  (3) five weeks after transplanting to the foliage.  Each plant received about 2 tbsp of the Vitazyme solution each time it was applied.

Fruit per cluster, and character:

Control: 3 (light fruit)
Vitazyme, 15 ml/gal: 3 (light fruit)

Vitazyme, 20 ml/gal: 4 (solid, juicy fruit)

Vitazyme, 30 ml/gal: 5 (blocky, juicy fruit)

 

Root and plant characteristics:

Vitazyme treatment increased fine root mass and produced longer roots, produced larger and darker green leaves, and thicker stems, more branches, better plant vigor, and many more flowers.

 

Average fruit weight (10 fruit):

Control: 86 grams
Vitazyme, 15 ml/gal: 86 grams
(0%)

Vitazyme, 20 ml/gal: 100 grams (+16%)

Vitazyme, 30 ml/gal: 117 grams (+36%)

Fertilization:  equal for all plots

 

 

 

Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

1999

Trinidad, West Indies (Caribbean Agricultural Research and Development Institute)

Variety:  Heatmaster

Transplanting date:  September 19, 1999

Vitazyme application:  A 1% Vitazyme solution was sprayed over the leaves and soil on 9/21, 10/6, and 10/24.

Determinations on November 10, 1999

(10 randomly selected plants from each treatment)

Plant height:

Control: 43.1 cm
Vitazyme: 45.3 cm*
(+6%)

*Significantly greater than the control at P=0.16.

 

Stem circumference:

Control: 1.7 cm
Vitazyme: 2.2 cm*
(+29%)

*Significantly greater than the control at P=0.001.

 

Fruit in first set:

Control: 3.3
Vitazyme: 3.9*
(+18%)

*Significantly greater than the control at P=0.017.

 

Side shoots:

Control: 4.0
Vitazyme: 4.1
(+3%)

 

Determinations on December 6, 1999

(three randomly selected plants from each treatment)

Plant height:

Control: 101 cm
Vitazyme: 125 cm
(+24%)

 

Root length:

Control: 16 cm
Vitazyme: 32 cm
(+100%)

 

Shoot number:

Control: 5
Vitazyme: 8
(+60%)

 

Stem diameter:

Control: 1.25 cm
Vitazyme: 2.00 cm
(+60%)

 

Fruit weight:

Control: 5.56 lb
Vitazyme: 11.50 lb
(+107%)

 

Fruit number:

Control: 18
Vitazyme: 34
(+89%)

 

Fruit per cluster:

Control: 3
Vitazyme: 4
(+33%)

Fertilization:  At transplanting, 15 g/plant in the planting hole of 12-24-12;  7 to 10 days later, same as above topdressed;  3 and 5 weeks after transplanting, 15 g/plant of a 12-12-17-2 (Mg);  7 and 10 weeks after transplanting, 15 g/plant of a 9-6-24.

 

 

 

Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Trinidad, West Indies (Caribbean Chemicals International)

Variety:  Gempride

Transplanting date:  November 3, 1999

Harvest date:  January 19, 2000

Vitazyme application:  Vitazyme was applied at 30 ml/gallon (about 1%) on the following dates:  November 10, November 25, December 9, and December 29, 1999.  These dates were 7, 22, 36, and 56 days after transplanting.  Each plant received about 2 tbsp. of the Vitazyme solution when it was applied.  The total application (four times) was 3.27 liters/hectare.

Early Pre-Harvest Data

Blossoms per plant:

Control: 19.1
Vitazyme: 28.7*
(+50%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Side shoots per plant:

Control: 7.9
Vitazyme: 9.2*
(+16%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Bract flowers per plant:

Control: 5.1
Vitazyme: 5.4
(+6%)

 

Fruit per plant:

Control: 19
Vitazyme: 45*
(+137%)

*Not significantly different due to the high variability of only two reps.

 

Leaf area at 8-in height:

Control: 3,823 mm2
Vitazyme: 6,674 mm2*
(+75%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Flowers per plant:

Control: 44
Vitazyme: 87*
(+98%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Final Harvest Data

Side shoots:

Control: 8.0
Vitazyme: 9.4
(+18%)

 

Plant height:

Control: 1.16 m
Vitazyme: 1.33 m*
(+15%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Stem circumference:

Control: 39 mm
Vitazyme: 46 mm*
(+18%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Fruit diameter:

Control: 6.64 cm
Vitazyme: 6.99 cm*
(+5%)

*Significantly greater than the control at P=0.05 (Tukey's Test).

 

Total fruit number:

Control: 554
Vitazyme: 980
(+77%)

 

Total fruit weight:

Control: 24.33 kg
Vitazyme: 45.79 kg
(+88%)

 

Average fruit weight:

Control: 43.9
Vitazyme: 46.7
(+6%)

 

Fruit flavor: A taste panel rated the Vitazyme treated fruit as superior to the controls.

Fertilization:  equal for all plots

 

 

 

Testimonial

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Hanford, California

Variety:  370's (plants)

Soil type:  sandy loam

Irrigation:  furrow

Vitazyme application:  13 oz/acre at planting, 13 oz/acre knifed in with the fertilizer

Comments:

The Vitazyme treated plants displayed superiority to the control plants during the season, with better color and size of the plantings.  At harvest the treated area was not kept totally separate from the control area, so accurate yield results were impossible.  However, the following observations on the Vitazyme treated tomatoes were made.

  • The fruit was bigger.

  • The color was more uniform.

  • The yield was likely one to two tons/acre greater ... possibly more.

  • The solids of the fruit were higher.

Fertilization:  according to soil test

 

 

 

Testimonial

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Fredonia, New York

Variety:  unknown

Soil type:  clay loam

Vitazyme application:  13 oz/acre in the transplant water, and two more 13 oz/acre applications with cover sprays, beginning at fruit set

Comments:

Comments made by the researcher:  "The yield was higher than usual.  Overall plant health was superior, and quality was consistent throughout the season.  This treated crop, although planted later than some other fields in the area, was the first to ripen.  Also, this crop continued to yield late in the season when others had quit, with good quality even late in the season."

Fertilization:  balanced fertilizer applications with regular cover sprays, especially with fungicide sprays after fruit set

 

 

 

 

 

 

Replicated Study (Rhizosphere Microbes)

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2000

Corvallis Oregon (Soil Foodweb, Inc.)

Varieties:  Heinz paste type, Celebrity, and Abe Lincoln

Planting date:  seeded April 5, and transplanted June 3, 2000

Soil type:  unknown

Row spacing:  36 inches

Planting rate:  unknown

Rhizosphere soil sampling dates:  June 4, June 12, July 3, July 12, and August 10, 2000

Replications: five (row sections about 15 feet long, randomly placed)

Vitazyme application:  200 ml/plant of a 0.5% Vitazyme solution on June 4, July 3, and August 3, 2000, over the root zone of appropriate plants

All analyses were performed at the Soil Foodweb laboratory in Corvallis, Oregon.  Rhizospere soil was used for all analyses.

Data for the last reading (August 10) are shown here.

Total bacterial biomass:

Control: 205 ug/g
Vitazyme: 234 ug/g
(+14%)

 

Active fungal biomass:

Control: 24.8 ug/g
Vitazyme: 44.1 ug/g
(+78%)

 

Total fungal biomass:

Control: 39.8 ug/g
Vitazyme: 57.4 ug/g
(+44%)

 

Flagellate protozoa biomass:

Control: 52.6 ug/g
Vitazyme: 134.5 ug/g
(+156%)

 

Total nematode numbers (mostly beneficials):

Control: 2.7/g
Vitazyme: 4.8/g
(+78%)

 

Mycorrhizal colonization (% of root cells infected):

Control: 9.8%
Vitazyme: 17.9%
(+83%)

 

Total fungal to total bacterial biomass:

Control: 0.20
Vitazyme: 0.25
(+25%)

 

Active fungal to active bacterial biomass:

Control: 2.49
Vitazyme: 4.43
(+78%)

 

Plant-available nitrogen supply from predators:

Control: 105 lb/acre of nitrogen
Vitazyme: 230 lb/acre of nitrogen
(+125 lb/acre of nitrogen)

 

Starting from inferior roots, the soil foodweb composition as affected by Vitazyme improved considerably over the tomato growing season.

Fertilization:  equal for all plants

 

 

 

Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Lemoorie, California

Variety:  Heinz 410, a round cannery processing type

Planting date:  first part of April

Soil type:  unknown

Row spacing:  60 inches, 14 inches in the row

Planting rate:  about 7,500 plants/acre

Harvest date:  August 2, 2001

Vitazyme application:  (1) Preplant, before transplanting, shanked in at 13 oz/acre 6 inches on either side of the rows, 2 inches above the furrow level;  (2) Sidedresed at 13 oz/acre in May, when UN-32 was applied at early blossom

Tomato color (deepness of red)*:

Control: 25.0
Vitazyme: 23.8**
(-1.2 points: deeper red color)

*Tomato color is evaluated by grinding the frui and evaluating redness of the internal flesh.  Green=30, Red=24 (the ideal color).

**Significantly different from the control at P=0.10, using a completely randomized design and the Tukey-Kramer Test.

 

Percent solids:

Control: 5.64%

Vitazyme: 5.74%* (+0.1%)

*Significantly different from the control at P=0.19, using a completely randomized design and the Tukey-Kramer Test.

Fertilization:  Fertilizers were applied according to a soil analysis.  Preplant:  500 lb per acre of 3-10-10+Zn (1 gal/acre). Sidedress:  150 lb/acre of UN-32.

 

 

 

Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2001

Hayang Eup, Kyungju City, Kyungbuk, Korea (Daegu University)

Variety:  House Doterang

Seeding date:  December 22, 2000

Soil type:  "market bed" soil

Transplanting date:  January 6, 2001

Seeding date:  December 22, 2000

Data collection:  Data were compiled on March 8, 2001.

Vitazyme application:  A 1:2,000 dilution (0.05%) was used in this study as a foliar spray on February 16 and 26, and March 6.

Plant height:

Control: 48.1 cm
Vitazyme: 48.6 cm
(+1%)

 

Fresh weight, above-ground portion:

Control: 55.0 grams
Vitazyme: 60.6 grams
(+10%)

 

Stem diameter:

Control: 6.46 cm
Vitazyme: 6.54 cm
(+1%)

 

Root dry weight:

Control: 0.74 grams
Vitazyme: 0.82 grams
(+11%)

Fertilization:  unknown

 

 

 

Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Santiago de Las Vegas, City of Havana Province, Cuba

(Research Institute of Tropical Agriculture Fundamentals: INIFAT)

Variety:  INIFAT-28, a salad tomato

Transplanting date:  February 25, 2003

Soil type:  red ferralitic

Previous crop:  unknown

Vitazyme applicationSeedlings:  For the Vitazyme plots the seedlings were inserted for 10 minutes in a jar containing 60 ml in 10 liters of water (a 0.6% solution) before planting.

Field:  A hand sprayer containing 50 ml of Vitazyme in 500 ml of water (a 1% solution) was used to apply to the leaves of the plants on March 12 (15 days after planting).  A second application was made by sprayer on April 1, 34 days after planting.

Flower number per plant, at 60 days:

Control: 4.2
Vitazyme: 6.2*
(+48%)

*Significantly greater than the control at P=0.05 according to Duncan's Multiple Range Test.

 

Fruit number per plant, at 60 days:

Control: 6.2
Vitazyme: 8.1*
(+31%)

*Significantly greater than the control at P=0.05 according to Duncan's Multiple Range Test.

 

Total fruit diameter:

Control: 6.27 cm
Vitazyme: 6.42 cm
(+2%)

 

Total fruit weight:

Control: 110.4 grams
Vitazyme: 113.0 grams
(+3%)

 

Total fruit per plot:

Control: 448 fruit/plot
Vitazyme: 576 fruit/plot
(+29%)

 

Yield per plot:

Control: 56.0 Kg of fruit/plot
Vitazyme: 65.0 Kg of fruit/plot
(+16%)

 

Total fruit number:

Control: 89,600
Vitazyme: 115,200
(+28%)

 

Total tomato yield:

Control: 11.0 tons
Vitazyme: 13.o tons
(+18%)

Fertilization:  standard for the institute

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Cuba

(INIFAT)

Variety:  INIFAT 28

Planting date:  unknown

Transplanting date:  unknown

Soil type:  Leptic haplustert

Vitazyme application:  unknown

Tomato yield:

Control: 11.0 tons/ha
Vitazyme: 13.0 tons/ha
(+18%)

 

Fruit number:

Control: 89,600
Vitazyme: 115,200
(+29%)

Fertilization:  unknown

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Dos Rios,

 Palma Soriano,

 Santiago de Cuba

Variety:  unknown

Planting date:  January 1, 2004

Transplanting date:  unknown

Soil type:  Leptic haplustert

Vitazyme application:  1 liter/ha twice, once at transplanting on January 20, 2004, and again on February 11, 2004

Fruit per plant (February 23, 2004):

Control: 21.4
Vitazyme: 32.4
(+42%)

 

Plant height (January 21, 2004):

Control: 18.0 cm
Vitazyme: 23.7 cm
(+32%)

 

Plant height (February 6, 2004):

Control: 38.9 cm
Vitazyme: 53.4 cm
(+15%)

 

Average fruit weight:

Control: 22.8 grams
Vitazyme: 36.8 grams
(+61%)

 

Estimated yield:

Control: 160 kg/plot
Vitazyme: 362.4 kg/plot
(+125%)

Fertilization:  unknown

 

 

 

Non-Replicated Study

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2004

Mayajigua, Sancti Spiritus, Cuba

Varieties:  Rome and Lignom

Planting date:  unknown

Plant spacing:  unknown

Soil type:  "gleyish" Vertisol

Vitazyme application:  1 liter/ha to the plants after the first picking

Lignom Tomatoes

Leaf number:

Control: 15
Vitazyme: 18
(+20%)

 

Rome Tomatoes

Fruit yield and color:

Control: 10 tons/ha; Light red color

Vitazyme: 15 tons/ha (+50%)  Dark red color

 

Fruit weight:

Control: 340 grams/fruit
Vitazyme: 453 grams/fruit
(+33%)

Fertilization:  compost only

 

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