Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Canton, Texas (AM&D Tree Farm)

Variety:  Live oak, Red oak, Magnolia

Potting soil type: custom mix using Vital Earth Resources raw materials

Planting date: all established trees in pots

Pot size: 45, 65, and 95 gallons

Irrigation: drip

Vitazyme application:  sprayed on the soil surface of each pot using a backpack sprayer, at 13 oz/acre directed only on the pot soil surfaces; made on April 5 and June 21, 2002

Evaluations on May 21, 46 days after appl. 1

Live oaks, golf course area (45 gallon pots):

Branch growth:

   Untreated: 7.4 cm

   Vitazyme once: 10.6 cm(+43%)

No leafing on branches:

   Untreated: 44%

   Vitazyme once: 14% (30 percentage points more)

Live oaks, north area (45 gallon pots):

Branch growth:

   Untreated: 8.9 cm

   Vitazyme once: 6.2 cm(+82%)

No leafing on branches:

   Untreated: 15%

   Vitazyme once: 3% (12 percentage points more)

Red oaks, golf course area (65 gallon pots):

Branch growth:

   Untreated: 24.9 cm

   Vitazyme once: 32.2 cm(+29%)

Magnolia (95 gallon pots):

Branch growth:

   Untreated: 31.1 cm

   Vitazyme once: 36.4 cm(+17%)

Evaluations on Sep. 10, 81 days after appl. 2

Live oaks, golf course area (45 gallon pots):

Branch growth:

   Untreated: 26.8 cm

   Vitazyme once: 38.8 cm(+41%)

Trunk caliper:

   Untreated: 2.31 inches

   Vitazyme once: 2.34 inches (+1.3%)

Live oaks, north area (45 gallon pots):

Branch growth:

   Untreated: 33.2 cm

   Vitazyme once: 40.6 cm(+22%)

Trunk caliper:

   Untreated: 2.24 inches

   Vitazyme once: 2.28 inches (+1.8%)

Red oaks, golf course area (65 gallon pots):

Branch growth:

   Untreated: 24.2 cm

   Vitazyme once: 32.9 cm(+36%)

Trunk caliper:

   Untreated: 1.91 inches

   Vitazyme once: 2.00 inches (+5%)

Magnolia (95 gallon pots):

Trunk caliper:

   Untreated: 2.77 inches

   Vitazyme once: 2.96 inches (+7%)

Fertilization: Different amounts of pelleted, coated fertilizer were applied in mid-March to each pot, the amount depending on the pot size, using a mixture of "Multicoat" and "Nutricoat"; 45 gallon pots received 300 grams, 65 gallons received 360 grams, and 95 gallon pots received 430 grams.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2002

Canton, Texas (AM&D Tree Farm)

Variety:  Savannah holly, Nellie R. Stevens holly, Foster holly

Potting soil type: custom mix using Vital Earth Resources raw materials

Planting date: all established trees in pots

Pot size: 3 and 65 gallons

Irrigation: drip and sprinkler

Vitazyme application:  sprayed on the soil surface of each pot using a backpack sprayer, at 13 oz/acre directed only on the pot soil surfaces; made on April 5 and June 21, 2002

Evaluations on May 21, 46 days after appl. 1

Savannah holly, large (65 gallon pots):

Branch growth:

   Untreated: 17.7 cm

   Vitazyme once: 20.4 cm(+15%)

Nellie R. Stevens holly, large (65 gallon pots):

Branch growth:

   Untreated: 41.0 cm

   Vitazyme once: 44.4 cm(+8%)

Nellie R. Stevens holly, small (3 gallon pots):

Branch growth:

   Untreated: 14.5 cm

   Vitazyme once: 15.8 cm(+9%)

Foster holly, small (3 gallon pots):

Branch growth:

   Untreated: 13.5 cm

   Vitazyme once: 15.4 cm(+14%)

Evaluations on Sep. 10, 81 days after appl. 2

Savannah holly, large (65 gallon pots):

Branch growth:

   Untreated: 18.3 cm

   Vitazyme once: 21.1 cm(+15%)

Nellie R. Stevens holly, large (65 gallon pots):

Branch growth:

   Untreated: 30.7 cm

   Vitazyme once: 35.7 cm(+16%)

Nellie R. Stevens holly, small (3 gallon pots):

Branch growth:

   Untreated: 16.7 cm

   Vitazyme once: 17.9 cm(+7%)

Foster holly, small (3 gallon pots):

Branch growth:

   Untreated: 15.6 cm

   Vitazyme once: 19.7 cm(+26%)

Fertilization: Different amounts of pelleted, coated fertilizer were applied in mid-March to each pot, the amount depending on the pot size, using a mixture of "Multicoat" and "Nutricoat"; 3 gallons received 40 grams, and 65 gallons received 360 grams.

Date

Location

Cultural System

Vitazyme/Fertilization

Results

2003

Diboll, Texas (Temple-Inland, Applied Research and Development)

Variety:  Loblolly pine

Soil type: deep coarse sand with <1% organic matter

Planting date: March, 2003

Irrigation: overhead sprinklers, irrigated daily

Fumigation: methyl bromide in October, 2002

Topping: Trees were cut off to a certain height before digging.

Growth analysis: lifted in December of 2002 and bagged for a few weeks before measuring

Replications: three

Vitazyme application:  (1) 13 oz/acre sprayed on all three Vitazyme treatments in late April, when seedlings were newly emerged; (2) 13 oz/acre sprayed over Treatments 2 and 3 in early June; (3) 13 oz/acre sprayed over Treatment 3 in early August

Root collar diameter*:

Untreated: 4.51 mm c

Vitazyme once: 4.79 mm ab (+6%)

Vitazyme twice: 4.64 mm bc (+3%)

Vitazyme three times: 4.98 mm a (+10%)

*Means followed by the same letter are not significantly different at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.01=0.29 mm.

Stem length*:

Untreated: 31.74 cm c

Vitazyme once: 35.15 cm ab (+11%)

Vitazyme twice: 34.09 cm b (+7%)

Vitazyme three times: 35.96 cm a (+13%)

*Means followed by the same letter are not significantly different at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.01=1.81 cm.

Root weight*:

Untreated: 0.984 grams a

Vitazyme once: 1.085 grams a (+10%)

Vitazyme twice: 1.002 grams a (+2%)

Vitazyme three times: 1.196 grams a (+22%)

*Means followed by the same letter are not significantly different at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.01=0.250.

Stem weight*:

Untreated: 4.209 cm b

Vitazyme once: 4.929 cm ab (+17%)

Vitazyme twice: 4.777 cm ab (+13%)

Vitazyme three times: 5.320 cm a (+26%)

*Means followed by the same letter are not significantly different at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.01=1.033 grams.

Root:Stem*:

Untreated: 0.2345 a

Vitazyme once: 0.2192 a (-7%)

Vitazyme twice: 0.2083 a (-11%)

Vitazyme three times: 0.2208 a (-6%)

*Means followed by the same letter are not significantly different at P=0.10 according to the Student-Newman-Keuls Test.  LSD_0.01=0.0463.

Fertilization: 150 lb/acre of N, in four to five applications beginning with a low rate and then "ramped up" as the trees grew.  The beds had received annual applications of composted bark mulch and green manure cover crops to try and increase organic matter.